Selection of target-binding proteins from the information of weakly enriched phage display libraries by deep sequencing and machine learning

Selection of target-binding proteins from the information of weakly enriched phage display libraries by deep sequencing and machine learning

ABSTRACTDespite the advances in surface-display systems for directed evolution, variants with high affinity are not always enriched due to undesirable biases that increase target-unrelated variants during biopanning. Here, our goal was to design a library containing improved variants from the inform...

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Bibliographic Details
Main Authors: Tomoyuki Ito, Thuy Duong Nguyen, Yutaka Saito, Yoichi Kurumida, Hikaru Nakazawa, Sakiya Kawada, Hafumi Nishi, Koji Tsuda, Tomoshi Kameda, Mitsuo Umetsu
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:mAbs
Subjects:
Machine learning
antibody mimetics
directed evolution
deep sequencing analysis
phage display
Online Access:https://www.tandfonline.com/doi/10.1080/19420862.2023.2168470

Internet

https://www.tandfonline.com/doi/10.1080/19420862.2023.2168470

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