Construction of a Derivative of Agrobacterium tumefaciens C58 That Does Not Mutate to Tetracycline Resistance
Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
The American Phytopathological Society
2001-01-01
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Series: | Molecular Plant-Microbe Interactions |
Subjects: | |
Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.1.98 |