Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white
An immobilized metal ion high-performance liquid chromatographic technique was developed for the determination of one of the egg allergens - ovomucoid - in egg white and food products containing egg white. The method was tested using standard samples as well as complete egg white and its reproducibi...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-06-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200203-0002_immobilized-metal-ion-chromatographic-imac-determination-of-ovomucoid-in-hen-s-egg-white.php |
| _version_ | 1797899855944744960 |
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| author | A. Prošková J. Kučera |
| author_facet | A. Prošková J. Kučera |
| author_sort | A. Prošková |
| collection | DOAJ |
| description | An immobilized metal ion high-performance liquid chromatographic technique was developed for the determination of one of the egg allergens - ovomucoid - in egg white and food products containing egg white. The method was tested using standard samples as well as complete egg white and its reproducibility was determined. The possible application of this method is discussed. The method is based on the HPLC chromatography on the chelating column saturated with copper ions. The sample is applied to the column at pH 7.5 and eluted isocraticaly with a buffer containing 1.75 mM imidazole in the optimum case. |
| first_indexed | 2024-04-10T08:36:34Z |
| format | Article |
| id | doaj.art-00b22f809f434f90bde2c8a525520a8a |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:36:34Z |
| publishDate | 2002-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-00b22f809f434f90bde2c8a525520a8a2023-02-23T03:26:36ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172002-06-01203959710.17221/3517-CJFScjf-200203-0002Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg whiteA. Prošková0J. Kučera1Food Research Institute Prague, Prague, Czech RepublicFood Research Institute Prague, Prague, Czech RepublicAn immobilized metal ion high-performance liquid chromatographic technique was developed for the determination of one of the egg allergens - ovomucoid - in egg white and food products containing egg white. The method was tested using standard samples as well as complete egg white and its reproducibility was determined. The possible application of this method is discussed. The method is based on the HPLC chromatography on the chelating column saturated with copper ions. The sample is applied to the column at pH 7.5 and eluted isocraticaly with a buffer containing 1.75 mM imidazole in the optimum case.https://cjfs.agriculturejournals.cz/artkey/cjf-200203-0002_immobilized-metal-ion-chromatographic-imac-determination-of-ovomucoid-in-hen-s-egg-white.phpovomucoiddeterminationhpl.cegg whiteanalytical methodimachplc |
| spellingShingle | A. Prošková J. Kučera Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white Czech Journal of Food Sciences ovomucoid determination hpl.c egg white analytical method imac hplc |
| title | Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white |
| title_full | Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white |
| title_fullStr | Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white |
| title_full_unstemmed | Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white |
| title_short | Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen's egg white |
| title_sort | immobilized metal ion chromatographic imac determination of ovomucoid in hen s egg white |
| topic | ovomucoid determination hpl.c egg white analytical method imac hplc |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200203-0002_immobilized-metal-ion-chromatographic-imac-determination-of-ovomucoid-in-hen-s-egg-white.php |
| work_keys_str_mv | AT aproskova immobilizedmetalionchromatographicimacdeterminationofovomucoidinhenseggwhite AT jkucera immobilizedmetalionchromatographicimacdeterminationofovomucoidinhenseggwhite |