Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection
This study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric a...
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| Format: | Article |
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MDPI AG
2010-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/11/11/4297/ |
| _version_ | 1811244608339836928 |
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| author | Jin Sun Yong-Hui Shi Ya-Xin Li Jie-Yun Wang Guo-Wei Le Yun-Jian Lin |
| author_facet | Jin Sun Yong-Hui Shi Ya-Xin Li Jie-Yun Wang Guo-Wei Le Yun-Jian Lin |
| author_sort | Jin Sun |
| collection | DOAJ |
| description | This study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric acid and/or citric acid). The highest degree of hydrolysis (DH) was 9.5% using 4 mol/L phosphoric acid with a ratio of 1:6 under a microwave intensity of 510 W for 240 s. Neutral proteinase gave higher DH among the four protease tested (Acid protease, neutral protease, Alcalase and papain), with an optimum condition of: (1) ratio of enzyme and substrate, 4760 U/g; (2) concentration of substrate, 4%; (3) reaction temperature, 55 °C and (4) pH 7.0. At 4 h, DH increased significantly (P < 0.01) under nitrogen protection compared with normal microwave assisted acid pre-treatment hydrolysis conditions. The antioxidant ability of the hydrolysate increased and reached its maximum value at 3 h; however DH decreased dramatically after 3 h. Microwave assisted acid pre-treatment and nitrogen protection could be a quick preparatory method for hydrolyzing bone collagen. |
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| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T14:28:10Z |
| publishDate | 2010-11-01 |
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| spelling | doaj.art-00cb136692414a8a9c8f376d2224ce002022-12-22T03:29:22ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-11-0111114297430810.3390/ijms11114297Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen ProtectionJin SunYong-Hui ShiYa-Xin LiJie-Yun WangGuo-Wei LeYun-Jian LinThis study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric acid and/or citric acid). The highest degree of hydrolysis (DH) was 9.5% using 4 mol/L phosphoric acid with a ratio of 1:6 under a microwave intensity of 510 W for 240 s. Neutral proteinase gave higher DH among the four protease tested (Acid protease, neutral protease, Alcalase and papain), with an optimum condition of: (1) ratio of enzyme and substrate, 4760 U/g; (2) concentration of substrate, 4%; (3) reaction temperature, 55 °C and (4) pH 7.0. At 4 h, DH increased significantly (P < 0.01) under nitrogen protection compared with normal microwave assisted acid pre-treatment hydrolysis conditions. The antioxidant ability of the hydrolysate increased and reached its maximum value at 3 h; however DH decreased dramatically after 3 h. Microwave assisted acid pre-treatment and nitrogen protection could be a quick preparatory method for hydrolyzing bone collagen.http://www.mdpi.com/1422-0067/11/11/4297/bone collagennitrogen protectionmicrowave assisted acid pre-treatmentenzyme hydrolysisantioxidant activity |
| spellingShingle | Jin Sun Yong-Hui Shi Ya-Xin Li Jie-Yun Wang Guo-Wei Le Yun-Jian Lin Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection International Journal of Molecular Sciences bone collagen nitrogen protection microwave assisted acid pre-treatment enzyme hydrolysis antioxidant activity |
| title | Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection |
| title_full | Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection |
| title_fullStr | Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection |
| title_full_unstemmed | Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection |
| title_short | Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection |
| title_sort | antioxidative peptides derived from enzyme hydrolysis of bone collagen after microwave assisted acid pre treatment and nitrogen protection |
| topic | bone collagen nitrogen protection microwave assisted acid pre-treatment enzyme hydrolysis antioxidant activity |
| url | http://www.mdpi.com/1422-0067/11/11/4297/ |
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