Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

Summary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enabl...

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Bibliographic Details
Main Authors: Bhaskar Jyoti Borah, Chi-Kuang Sun
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002106