Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
Summary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enabl...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002106 |
| _version_ | 1817984465378476032 |
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| author | Bhaskar Jyoti Borah Chi-Kuang Sun |
| author_facet | Bhaskar Jyoti Borah Chi-Kuang Sun |
| author_sort | Bhaskar Jyoti Borah |
| collection | DOAJ |
| description | Summary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process.For complete details on the use and execution of this protocol, please refer to Borah et al. (2021). |
| first_indexed | 2024-04-13T23:44:58Z |
| format | Article |
| id | doaj.art-00e29840ae1241e3afea3274aced7270 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T23:44:58Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-00e29840ae1241e3afea3274aced72702022-12-22T02:24:21ZengElsevierSTAR Protocols2666-16672022-06-0132101330Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanningBhaskar Jyoti Borah0Chi-Kuang Sun1Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan; Corresponding authorDepartment of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan; Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan; Corresponding authorSummary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process.For complete details on the use and execution of this protocol, please refer to Borah et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722002106Cell BiologyMicroscopyBiotechnology and bioengineering |
| spellingShingle | Bhaskar Jyoti Borah Chi-Kuang Sun Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning STAR Protocols Cell Biology Microscopy Biotechnology and bioengineering |
| title | Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning |
| title_full | Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning |
| title_fullStr | Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning |
| title_full_unstemmed | Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning |
| title_short | Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning |
| title_sort | construction of a high nfom multiphoton microscope with large angle resonant raster scanning |
| topic | Cell Biology Microscopy Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722002106 |
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