In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells

In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells

Summary: Wilms’ tumor protein 1 (WT1) is a tumor-associated antigen overexpressed in various cancers. As a self-antigen, negative selection reduces the number of WT1-specific T cell receptors (TCRs). Here, we provide a protocol to generate WT137-45-specific TCRs using healthy human peripheral blood...

Full description

Bibliographic Details
Main Authors: Sanne van der Heijden, Donovan Flumens, Maarten Versteven, Stefanie Peeters, Hans De Reu, Diana Campillo-Davo, Yannick Willemen, Benson Ogunjimi, Viggo Van Tendeloo, Zwi N. Berneman, Sébastien Anguille, Evelien Smits, Eva Lion
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Culture
Cell Isolation
Flow Cytometry/Mass Cytometry
Cancer
Health Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000114

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723000114

Similar Items