X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion wi...

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Bibliographic Details
Main Authors: Christopher D. M. Hutchison, Violeta Cordon-Preciado, Rhodri M. L. Morgan, Takanori Nakane, Josie Ferreira, Gabriel Dorlhiac, Alvaro Sanchez-Gonzalez, Allan S. Johnson, Ann Fitzpatrick, Clyde Fare, Jon P. Marangos, Chun Hong Yoon, Mark S. Hunter, Daniel P. DePonte, Sébastien Boutet, Shigeki Owada, Rie Tanaka, Kensuke Tono, So Iwata, Jasper J. van Thor
Format: Article
Language:English
Published: MDPI AG 2017-09-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/18/9/1918