Efficient CRISPR-Cas9 Gene Disruption System in Edible-Medicinal Mushroom Cordyceps militaris

Cordyceps militaris is a well-known edible medicinal mushroom in East Asia that contains abundant and diverse bioactive compounds. Since traditional genome editing systems in C. militaris were inefficient and complicated, here, we show that the codon-optimized cas9, which was used with the newly rep...

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Bibliographic Details
Main Authors: Bai-Xiong Chen, Tao Wei, Zhi-Wei Ye, Fan Yun, Lin-Zhi Kang, Hong-Biao Tang, Li-Qiong Guo, Jun-Fang Lin
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.01157/full