MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway.
Major histocompatibility complex class II (MHC II) expression and turn-over are regulated via its ubiquitination by the membrane associated RING-CH 1 (MARCH1) E3 ligase. Unexpectedly, we show that MHC II ubiquitination also impacts MHC I. Lack of MARCH1 in B cells and dendritic cells (DCs) resulted...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6042767?pdf=render |
| _version_ | 1811242868893810688 |
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| author | Kayla R Wilson Haiyin Liu Geraldine Healey Vivian Vuong Satoshi Ishido Marco J Herold Jose A Villadangos Justine D Mintern |
| author_facet | Kayla R Wilson Haiyin Liu Geraldine Healey Vivian Vuong Satoshi Ishido Marco J Herold Jose A Villadangos Justine D Mintern |
| author_sort | Kayla R Wilson |
| collection | DOAJ |
| description | Major histocompatibility complex class II (MHC II) expression and turn-over are regulated via its ubiquitination by the membrane associated RING-CH 1 (MARCH1) E3 ligase. Unexpectedly, we show that MHC II ubiquitination also impacts MHC I. Lack of MARCH1 in B cells and dendritic cells (DCs) resulted in a significant reduction in surface MHC I expression. This decrease was not directly caused by changes in MARCH1 ubiquitination of MHC I but indirectly by altered MHC II trafficking in the absence of its ubiquitination. Deletion of MHC II in March1-/- cells restored normal MHC I surface expression and replacement of wild type MHC II by a variant that could not be ubiquitinated caused a reduction in MHC I expression. Furthermore, these cells displayed inefficient presentation of peptide and protein antigen via MHC I to CD8+ T cells. In summary, we describe an unexpected intersection between MHC I and MHC II such that the surface expression of both molecules are indirectly and directly regulated by MARCH1 ubiquitination, respectively. |
| first_indexed | 2024-04-12T13:57:24Z |
| format | Article |
| id | doaj.art-0121d50d5ca04b89820fd88555ba7ba3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T13:57:24Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0121d50d5ca04b89820fd88555ba7ba32022-12-22T03:30:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020054010.1371/journal.pone.0200540MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway.Kayla R WilsonHaiyin LiuGeraldine HealeyVivian VuongSatoshi IshidoMarco J HeroldJose A VilladangosJustine D MinternMajor histocompatibility complex class II (MHC II) expression and turn-over are regulated via its ubiquitination by the membrane associated RING-CH 1 (MARCH1) E3 ligase. Unexpectedly, we show that MHC II ubiquitination also impacts MHC I. Lack of MARCH1 in B cells and dendritic cells (DCs) resulted in a significant reduction in surface MHC I expression. This decrease was not directly caused by changes in MARCH1 ubiquitination of MHC I but indirectly by altered MHC II trafficking in the absence of its ubiquitination. Deletion of MHC II in March1-/- cells restored normal MHC I surface expression and replacement of wild type MHC II by a variant that could not be ubiquitinated caused a reduction in MHC I expression. Furthermore, these cells displayed inefficient presentation of peptide and protein antigen via MHC I to CD8+ T cells. In summary, we describe an unexpected intersection between MHC I and MHC II such that the surface expression of both molecules are indirectly and directly regulated by MARCH1 ubiquitination, respectively.http://europepmc.org/articles/PMC6042767?pdf=render |
| spellingShingle | Kayla R Wilson Haiyin Liu Geraldine Healey Vivian Vuong Satoshi Ishido Marco J Herold Jose A Villadangos Justine D Mintern MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. PLoS ONE |
| title | MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. |
| title_full | MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. |
| title_fullStr | MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. |
| title_full_unstemmed | MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. |
| title_short | MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. |
| title_sort | march1 mediated ubiquitination of mhc ii impacts the mhc i antigen presentation pathway |
| url | http://europepmc.org/articles/PMC6042767?pdf=render |
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