Quantifying F-actin patches in single melanoma cells using total-internal reflection fluorescence microscopy
Abstract Total-internal reflection fluorescence (TIRF) microscope is a unique technique for selective excitation of only those fluorophore molecules in a cellular environment, which are located at the sub-diffraction axial distance of a cell’s contact-area. Despite this prominent feature of the TIRF...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2022-11-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-022-22632-z |