Quantifying F-actin patches in single melanoma cells using total-internal reflection fluorescence microscopy

Abstract Total-internal reflection fluorescence (TIRF) microscope is a unique technique for selective excitation of only those fluorophore molecules in a cellular environment, which are located at the sub-diffraction axial distance of a cell’s contact-area. Despite this prominent feature of the TIRF...

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Bibliographic Details
Main Authors: Elham Sheykhi, Behnaz Shojaedin-Givi, Batool Sajad, Hossein Naderi-Manesh, Sharareh Tavaddod
Format: Article
Language:English
Published: Nature Portfolio 2022-11-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-22632-z