Optimization of the Conditions for Extraction of Serine Protease from Kesinai Plant (Streblus asper) Leaves Using Response Surface Methodology

Optimization of the Conditions for Extraction of Serine Protease from Kesinai Plant (Streblus asper) Leaves Using Response Surface Methodology

Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X1), mixing time (2–6 min, X2), buffer content...

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Bibliographic Details
Main Authors: Md. Zaidul Islam Sarker, Abdul Manap Mohd Yazid, Shuhaimi Mustafa, Amid Mehrnoush
Format: Article
Language:English
Published: MDPI AG 2011-11-01
Series:Molecules
Subjects:
extraction
enzyme
specific activity
stability
oxidizing agent
Online Access:http://www.mdpi.com/1420-3049/16/11/9245/

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http://www.mdpi.com/1420-3049/16/11/9245/

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