Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics

Emerging customized designs to upgrade the functional potential of freeze-dried apple pomace was used in this study, in order to transform the industrial by-products into ingredients containing probiotics, for a better and healthier food composition. The freeze-dried apple pomace was analyzed for fr...

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Main Authors: Camelia Cristina Vlad, Bogdan Păcularu-Burada, Aida Mihaela Vasile, Ștefania Adelina Milea, Gabriela-Elena Bahrim, Gabriela Râpeanu, Nicoleta Stănciuc
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/11/10/2028
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author Camelia Cristina Vlad
Bogdan Păcularu-Burada
Aida Mihaela Vasile
Ștefania Adelina Milea
Gabriela-Elena Bahrim
Gabriela Râpeanu
Nicoleta Stănciuc
author_facet Camelia Cristina Vlad
Bogdan Păcularu-Burada
Aida Mihaela Vasile
Ștefania Adelina Milea
Gabriela-Elena Bahrim
Gabriela Râpeanu
Nicoleta Stănciuc
author_sort Camelia Cristina Vlad
collection DOAJ
description Emerging customized designs to upgrade the functional potential of freeze-dried apple pomace was used in this study, in order to transform the industrial by-products into ingredients containing probiotics, for a better and healthier food composition. The freeze-dried apple pomace was analyzed for free and bounded phenolic contents, highlighting a significant level of caffeic acid (4978.00 ± 900.00 mg/100 g dry matter (DM)), trans-cinnamic acid (2144.20 ± 37.60 mg/100 g DM) and quercetin 3-β-D-glucoside (236.60 ± 3.12 mg/100 g DM). The pectin extraction yield was approximatively 24%, with a degree of esterification of 37.68 ± 1.74%, and a methoxyl content of 5.58 ± 0.88%. The freeze-dried apple pomace was added in a different ratio as a supplement to cultural medium of <i>Loigolactobacillus bifermentans</i> MIUG BL 16, suggesting a significant prebiotic effect (<i>p</i> < 0.05) at concentration between 1% and 2%. The apple pomace was used to design three freeze-dried ingredients containing probiotic, with a high level of polyphenolic content (6.38 ± 0.14 mg gallic acid equivalents/g DM) and antioxidant activity (42.25 ± 4.58 mMol Trolox/g DM) for the powder containing apple pomace ethanolic extract. When inulin was used as a prebiotic adjuvant, the obtained powder showed a 6 log/g DM viable cell count. The ingredients were added to fermented vegetable soy milk-based products, allowing us to improve the polyphenolic content, antioxidant activity and viable cell counts. The approach designed in this study allowed us to obtain ingredients suitable to add value to food, whereas premises to align with the current circular economy premises, by reintegrating the industrial waste as sources of high added value compounds, are also provided.
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spelling doaj.art-01551cba2712493a993cc6fc504a526b2023-11-23T22:39:36ZengMDPI AGAntioxidants2076-39212022-10-011110202810.3390/antiox11102028Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with ProbioticsCamelia Cristina Vlad0Bogdan Păcularu-Burada1Aida Mihaela Vasile2Ștefania Adelina Milea3Gabriela-Elena Bahrim4Gabriela Râpeanu5Nicoleta Stănciuc6Faculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Dunărea de Jos University of Galati, Domnească Street 111, 800201 Galati, RomaniaEmerging customized designs to upgrade the functional potential of freeze-dried apple pomace was used in this study, in order to transform the industrial by-products into ingredients containing probiotics, for a better and healthier food composition. The freeze-dried apple pomace was analyzed for free and bounded phenolic contents, highlighting a significant level of caffeic acid (4978.00 ± 900.00 mg/100 g dry matter (DM)), trans-cinnamic acid (2144.20 ± 37.60 mg/100 g DM) and quercetin 3-β-D-glucoside (236.60 ± 3.12 mg/100 g DM). The pectin extraction yield was approximatively 24%, with a degree of esterification of 37.68 ± 1.74%, and a methoxyl content of 5.58 ± 0.88%. The freeze-dried apple pomace was added in a different ratio as a supplement to cultural medium of <i>Loigolactobacillus bifermentans</i> MIUG BL 16, suggesting a significant prebiotic effect (<i>p</i> < 0.05) at concentration between 1% and 2%. The apple pomace was used to design three freeze-dried ingredients containing probiotic, with a high level of polyphenolic content (6.38 ± 0.14 mg gallic acid equivalents/g DM) and antioxidant activity (42.25 ± 4.58 mMol Trolox/g DM) for the powder containing apple pomace ethanolic extract. When inulin was used as a prebiotic adjuvant, the obtained powder showed a 6 log/g DM viable cell count. The ingredients were added to fermented vegetable soy milk-based products, allowing us to improve the polyphenolic content, antioxidant activity and viable cell counts. The approach designed in this study allowed us to obtain ingredients suitable to add value to food, whereas premises to align with the current circular economy premises, by reintegrating the industrial waste as sources of high added value compounds, are also provided.https://www.mdpi.com/2076-3921/11/10/2028apple pomaceprobioticsprebioticspectinvalue-addedpolyphenols
spellingShingle Camelia Cristina Vlad
Bogdan Păcularu-Burada
Aida Mihaela Vasile
Ștefania Adelina Milea
Gabriela-Elena Bahrim
Gabriela Râpeanu
Nicoleta Stănciuc
Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics
Antioxidants
apple pomace
probiotics
prebiotics
pectin
value-added
polyphenols
title Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics
title_full Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics
title_fullStr Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics
title_full_unstemmed Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics
title_short Upgrading the Functional Potential of Apple Pomace in Value-Added Ingredients with Probiotics
title_sort upgrading the functional potential of apple pomace in value added ingredients with probiotics
topic apple pomace
probiotics
prebiotics
pectin
value-added
polyphenols
url https://www.mdpi.com/2076-3921/11/10/2028
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