Research on Improving the Catalytic Activity of Thermoacidophilic Type III Pullulan Hydrolase TK-PUL by Error-prone PCR

Research on Improving the Catalytic Activity of Thermoacidophilic Type III Pullulan Hydrolase TK-PUL by Error-prone PCR

Thermoacidophilic type III pullulan hydrolase TK-PUL can completely hydrolyze starch to starch sugar under liquefaction condition. It has great application potential in the ''one step liquefaction-saccharification'' starch syrup production process. In this study, error-prone PCR...

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Bibliographic Details
Main Authors: Jing ZENG, Jianjun GUO, Lin YUAN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2022-09-01
Series:Shipin gongye ke-ji
Subjects:
type iii pullulan hydrolase
directed evolution
error-prone polymerase chain reaction (pcr)
high-throughput screening technology
catalytic activity
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120091

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http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120091

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