Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform
Abstract Preclinical cancer research ranges from in vitro studies that are inexpensive and not necessarily reflective of the tumor microenvironment to mouse studies that are better models but prohibitively expensive at scale. Chorioallantoic membrane (CAM) assays utilizing Japanese quail (Coturnix j...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-02509-3 |
| _version_ | 1819037120087457792 |
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| author | Samuel V. Rasmussen Noah E. Berlow Lisa Hudson Price Atiya Mansoor Stefano Cairo Sandra Rugonyi Charles Keller |
| author_facet | Samuel V. Rasmussen Noah E. Berlow Lisa Hudson Price Atiya Mansoor Stefano Cairo Sandra Rugonyi Charles Keller |
| author_sort | Samuel V. Rasmussen |
| collection | DOAJ |
| description | Abstract Preclinical cancer research ranges from in vitro studies that are inexpensive and not necessarily reflective of the tumor microenvironment to mouse studies that are better models but prohibitively expensive at scale. Chorioallantoic membrane (CAM) assays utilizing Japanese quail (Coturnix japonica) are a cost-effective screening method to precede and minimize the scope of murine studies for anti-cancer efficacy and drug toxicity. To increase the throughput of CAM assays we have built and optimized an 11-day platform for processing up to 200 quail eggs per screening to evaluate drug efficacy and drug toxicity caused by a therapeutic. We demonstrate ex ovo concordance with murine in vivo studies, even when the in vitro and in vivo studies diverge, suggesting a role for this quail shell-free CAM xenograft assay in the validation of new anti-cancer agents. |
| first_indexed | 2024-12-21T08:16:21Z |
| format | Article |
| id | doaj.art-01643ff53c1b43ab8f9b56e7125517f1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T08:16:21Z |
| publishDate | 2021-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-01643ff53c1b43ab8f9b56e7125517f12022-12-21T19:10:32ZengNature PortfolioScientific Reports2045-23222021-12-011111810.1038/s41598-021-02509-3Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platformSamuel V. Rasmussen0Noah E. Berlow1Lisa Hudson Price2Atiya Mansoor3Stefano Cairo4Sandra Rugonyi5Charles Keller6Children’s Cancer Therapy Development InstituteChildren’s Cancer Therapy Development InstituteChildren’s Cancer Therapy Development InstituteChildren’s Cancer Therapy Development InstituteChildren’s Cancer Therapy Development InstituteDepartment of Biomedical Engineering, Oregon Health and Science UniversityChildren’s Cancer Therapy Development InstituteAbstract Preclinical cancer research ranges from in vitro studies that are inexpensive and not necessarily reflective of the tumor microenvironment to mouse studies that are better models but prohibitively expensive at scale. Chorioallantoic membrane (CAM) assays utilizing Japanese quail (Coturnix japonica) are a cost-effective screening method to precede and minimize the scope of murine studies for anti-cancer efficacy and drug toxicity. To increase the throughput of CAM assays we have built and optimized an 11-day platform for processing up to 200 quail eggs per screening to evaluate drug efficacy and drug toxicity caused by a therapeutic. We demonstrate ex ovo concordance with murine in vivo studies, even when the in vitro and in vivo studies diverge, suggesting a role for this quail shell-free CAM xenograft assay in the validation of new anti-cancer agents.https://doi.org/10.1038/s41598-021-02509-3 |
| spellingShingle | Samuel V. Rasmussen Noah E. Berlow Lisa Hudson Price Atiya Mansoor Stefano Cairo Sandra Rugonyi Charles Keller Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform Scientific Reports |
| title | Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform |
| title_full | Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform |
| title_fullStr | Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform |
| title_full_unstemmed | Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform |
| title_short | Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform |
| title_sort | preclinical therapeutics ex ovo quail eggs as a biomimetic automation ready xenograft platform |
| url | https://doi.org/10.1038/s41598-021-02509-3 |
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