HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids
Using high performance liquid chromatography-mass spectrometry(HPLC-MS/MS) technology, the components in the water extract of loquat leaves were analyzed, and the terpenoids were analyzed. Using a C18 column (2.1 mm×100 mm, 1.8 μm), using water +0.1% formic acid solution as mobile phase gradient elu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021040180 |
| _version_ | 1797984798470307840 |
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| author | Jiamei LI Wei XU Xue ZHANG Baiqi SHAO Hongyao XIE Fan WU Zhiyu MA Hua CHEN |
| author_facet | Jiamei LI Wei XU Xue ZHANG Baiqi SHAO Hongyao XIE Fan WU Zhiyu MA Hua CHEN |
| author_sort | Jiamei LI |
| collection | DOAJ |
| description | Using high performance liquid chromatography-mass spectrometry(HPLC-MS/MS) technology, the components in the water extract of loquat leaves were analyzed, and the terpenoids were analyzed. Using a C18 column (2.1 mm×100 mm, 1.8 μm), using water +0.1% formic acid solution as mobile phase gradient elution, using ESI ion source, collecting mass spectrometry data in positive and negative ion modes, a total of 120 compounds were detected ; Including 18 kinds of terpenoids with a total relative content of 1452.693 μg/mL; 24 kinds of flavonoids with a total relative content of 10378.081 μg/mL; 18 kinds of phenolic compounds with a total relative content of 36967.955 μg/mL; 5 kinds of coumarins compounds with a total relative content of 214.778 μg/mL; The structures of 18 terpenoids were further identified, including 6 ursane type, 7 oleanane type, 3 sesquiterpenes, and 1 diterpenoid. And a special iridoid glycosides; Roburic acid, ginkgolide B, prespatane, shanzhiside methyl ester, icariside B5, quillaic acid, asiatic acid, 18β-glycyrrhetinic acid were no report in the previous active substances of loquat leaves. This article provides a reference for the research on the water-extracted components of loquat leaves. |
| first_indexed | 2024-04-11T07:08:31Z |
| format | Article |
| id | doaj.art-017d3c9c3eb44a96939b8c5f92fb78a5 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T07:08:31Z |
| publishDate | 2022-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-017d3c9c3eb44a96939b8c5f92fb78a52022-12-22T04:38:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-01-0143129530310.13386/j.issn1002-0306.20210401802021040180-1HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of TerpenoidsJiamei LI0Wei XU1Xue ZHANG2Baiqi SHAO3Hongyao XIE4Fan WU5Zhiyu MA6Hua CHEN7School of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150076, ChinaNantong Tianlai Village Agricultural Science and Technology Development Co., Ltd., Haimen 226100, ChinaUsing high performance liquid chromatography-mass spectrometry(HPLC-MS/MS) technology, the components in the water extract of loquat leaves were analyzed, and the terpenoids were analyzed. Using a C18 column (2.1 mm×100 mm, 1.8 μm), using water +0.1% formic acid solution as mobile phase gradient elution, using ESI ion source, collecting mass spectrometry data in positive and negative ion modes, a total of 120 compounds were detected ; Including 18 kinds of terpenoids with a total relative content of 1452.693 μg/mL; 24 kinds of flavonoids with a total relative content of 10378.081 μg/mL; 18 kinds of phenolic compounds with a total relative content of 36967.955 μg/mL; 5 kinds of coumarins compounds with a total relative content of 214.778 μg/mL; The structures of 18 terpenoids were further identified, including 6 ursane type, 7 oleanane type, 3 sesquiterpenes, and 1 diterpenoid. And a special iridoid glycosides; Roburic acid, ginkgolide B, prespatane, shanzhiside methyl ester, icariside B5, quillaic acid, asiatic acid, 18β-glycyrrhetinic acid were no report in the previous active substances of loquat leaves. This article provides a reference for the research on the water-extracted components of loquat leaves.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021040180loquat leaveswater-extracted componentshplc-ms/msterpenoidsstructure identification |
| spellingShingle | Jiamei LI Wei XU Xue ZHANG Baiqi SHAO Hongyao XIE Fan WU Zhiyu MA Hua CHEN HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids Shipin gongye ke-ji loquat leaves water-extracted components hplc-ms/ms terpenoids structure identification |
| title | HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids |
| title_full | HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids |
| title_fullStr | HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids |
| title_full_unstemmed | HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids |
| title_short | HPLC-MS/MS Analysis of Water-Extracted Components of Loquat Leaves and Structural Identification of Terpenoids |
| title_sort | hplc ms ms analysis of water extracted components of loquat leaves and structural identification of terpenoids |
| topic | loquat leaves water-extracted components hplc-ms/ms terpenoids structure identification |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021040180 |
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