EXPERT expands prime editing efficiency and range of large fragment edits

EXPERT expands prime editing efficiency and range of large fragment edits

Abstract Prime editing systems (PEs) hold great promise in modern biotechnology. However, their editing range is limited as PEs can only modify the downstream sequences of the pegRNA nick. Here, we report the development of the extended prime editor system (EXPERT) to overcome this limitation by usi...

Full description

Bibliographic Details
Main Authors:	Youcai Xiong, Yinyu Su, Ruigao He, Xiaosong Han, Sheng Li, Minghuan Liu, Xiaoning Xi, Zijia Liu, Heng Wang, Shengsong Xie, Xuewen Xu, Kui Li, Jifeng Zhang, Jie Xu, Xinyun Li, Shuhong Zhao, Jinxue Ruan
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-02-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-025-56734-9

Similar Items

Development of Plant Prime-Editing Systems for Precise Genome Editing
by: Rongfang Xu, et al.
Published: (2020-05-01)

Enhancing the antibacterial activities of sow milk via site-specific knock-in of a lactoferrin gene in pigs using CRISPR/Cas9 technology
by: Xiaosong Han, et al.
Published: (2020-11-01)

Base editing and prime editing in laboratory animals
by: Caso, F, et al.
Published: (2021)

Plant genome editing: CRISPR, base editing, prime editing, and beyond
by: Yujie Xie, et al.
Published: (2022-12-01)

Current advancement in the application of prime editing
by: Zhangrao Huang, et al.
Published: (2023-02-01)

Efficient Gene Editing at Major CFTR Mutation Loci
by: Jinxue Ruan, et al.
Published: (2019-06-01)

Development and Application of Prime Editing in Plants
by: Liu Tingting, et al.
Published: (2023-11-01)

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants
by: Abdullah, et al.
Published: (2020-12-01)

HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing
by: Nan Liu, et al.
Published: (2022-09-01)

HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing
by: Nan Liu, et al.
Published: (2022-12-01)

From bench to bedside: cutting-edge applications of base editing and prime editing in precision medicine
by: Weihui Xu, et al.
Published: (2024-12-01)

The law of contracts (expanded edition)/
by: 294849 Wincor, Richard
Published: (1970)

Conserved A-to-I RNA editing with non-conserved recoding expands the candidates of functional editing sites
by: Yuange Duan, et al.
Published: (2024-12-01)

Prime time for base editing in the mitochondria
by: Michael A. Morgan, et al.
Published: (2022-07-01)

Prime Editing for Inherited Retinal Diseases
by: Bruna Lopes da Costa, et al.
Published: (2021-11-01)

Efficient Rescue of Retinal Degeneration in Pde6a Mice by Engineered Base Editing and Prime Editing
by: Zhiquan Liu, et al.
Published: (2024-11-01)

Advances in CRISPR-Based Functional Genomics and Nucleic Acid Detection in Pigs
by: Jinxue Ruan, et al.
Published: (2022-05-01)

The route of Parmenides. Revised and expanded edition
by: Marcelo Marques
Published: (2009-06-01)

Efficient and versatile multiplex prime editing in hexaploid wheat
by: Pei Ni, et al.
Published: (2023-06-01)

Peptide fusion improves prime editing efficiency
by: Minja Velimirovic, et al.
Published: (2022-06-01)

Base and Prime Editing Technologies for Blood Disorders
by: Panagiotis Antoniou, et al.
Published: (2021-01-01)

CRISPR/Cas9 or prime editing? – It depends on…
by: Schenke Dirk
Published: (2020-12-01)

PrimeDesign software for rapid and simplified design of prime editing guide RNAs
by: Jonathan Y. Hsu, et al.
Published: (2021-02-01)

WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing
by: Rui Tao, et al.
Published: (2022-04-01)

Explore the dominant factor in prime editing via a view of DNA processing
by: Zhimeng Xu, et al.
Published: (2023-09-01)

Efficient and in situ correction of hemoglobin Constant Spring mutation by prime editing in human hematopoietic cells
by: Congwen Shao, et al.
Published: (2024-12-01)

Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells
by: Zhengzheng Jiang, et al.
Published: (2022-03-01)

Mismatch prime editing gRNA increased efficiency and reduced indels
by: Jidong Fei, et al.
Published: (2025-01-01)

Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells
by: Mu Li, et al.
Published: (2022-10-01)

Prime Editing: Mechanistic Insights and DNA Repair Modulation
by: Astrid Mentani, et al.
Published: (2025-02-01)

Expanding C–T base editing toolkit with diversified cytidine deaminases
by: Tian-Lin Cheng, et al.
Published: (2019-08-01)

Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
by: Li Wang, et al.
Published: (2021-03-01)

Prime Editing, a Novel Genome-Editing Tool That May Surpass Conventional CRISPR-Cas9
by: Adriana Ochoa-Sanchez, et al.
Published: (2021-01-01)

Prime Editing: Genome Editing for Rare Genetic Diseases Without Double-Strand Breaks or Donor DNA
by: Ianis G. Matsoukas, et al.
Published: (2020-06-01)

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope
by: Xu Feng, et al.
Published: (2024-04-01)

Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis
by: Zhaoying Shi, et al.
Published: (2022-07-01)

Delivery of CRISPR/Cas9 Plasmid DNA by Hyperbranched Polymeric Nanoparticles Enables Efficient Gene Editing
by: Kemao Xiu, et al.
Published: (2022-12-01)

Efficient and precise generation of Tay–Sachs disease model in rabbit by prime editing system
by: Yuqiang Qian, et al.
Published: (2021-07-01)

Random-PE: an efficient integration of random sequences into mammalian genome by prime editing
by: Yaoge Jiao, et al.
Published: (2021-11-01)

MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
by: Linyuan Ma, et al.
Published: (2020-11-01)