Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant
Abstract The destruction of the pulmonary epithelial barrier in acute respiratory distress syndrome is caused by the damage of the alveolar epithelial cells. Oroxin A is an effective flavonoid component derived from the medicinal plant Oroxylum indicum (L.) Kurz. In this study, the oleic acid (OA)-i...
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Academia Brasileira de Ciências
2022-12-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000800601&tlng=en |
| _version_ | 1811185837700808704 |
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| author | JIAN HUANG CHEN CHEN JIANQIN XIE KERONG ZHAI SHILIN WEI XINGDONG CHENG RONGZHI ZHANG |
| author_facet | JIAN HUANG CHEN CHEN JIANQIN XIE KERONG ZHAI SHILIN WEI XINGDONG CHENG RONGZHI ZHANG |
| author_sort | JIAN HUANG |
| collection | DOAJ |
| description | Abstract The destruction of the pulmonary epithelial barrier in acute respiratory distress syndrome is caused by the damage of the alveolar epithelial cells. Oroxin A is an effective flavonoid component derived from the medicinal plant Oroxylum indicum (L.) Kurz. In this study, the oleic acid (OA)-induced A549 cell injury model was established in vitro to explore the protective mechanism of Oroxin A. The experiment was divided into the following groups: control, OA and OA + Oroxin A group. The OA-induced A549 cell injury was dose (time)-dependent and was detected by the CCK-8 assay. The protein and mRNA expression levels associated with pyroptosis are detected by Western blotting and RT-qPCR. After Oroxin A treatment, the levels of IL-1β, IL-18 and LDH released were significantly lower than the OA group. In terms of pyroptosis, Oroxin A can inhibit the expression of pyroptosis-related protein and mRNA. Significantly, the surfactant protein C (SPC) level in the OA + Oroxin A group was significantly higher than that in the OA group. The treatment with Oroxin A alleviates the OA-induced injury in the A549 cells, and its mechanism may be related to the inhibition of A549 cells pyroptosis and prevention of the degradation of the SPC. |
| first_indexed | 2024-04-11T13:36:04Z |
| format | Article |
| id | doaj.art-0199606b3e144d6e8536c87bbe499a4b |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T13:36:04Z |
| publishDate | 2022-12-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-0199606b3e144d6e8536c87bbe499a4b2022-12-22T04:21:28ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902022-12-0194suppl 410.1590/0001-3765202220211400Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactantJIAN HUANGhttps://orcid.org/0000-0002-8767-2904CHEN CHENhttps://orcid.org/0000-0002-4720-011XJIANQIN XIEhttps://orcid.org/0000-0001-9447-2639KERONG ZHAIhttps://orcid.org/0000-0002-6017-5668SHILIN WEIhttps://orcid.org/0000-0002-0596-2888XINGDONG CHENGhttps://orcid.org/0000-0001-6944-3107RONGZHI ZHANGhttps://orcid.org/0000-0002-7813-7715Abstract The destruction of the pulmonary epithelial barrier in acute respiratory distress syndrome is caused by the damage of the alveolar epithelial cells. Oroxin A is an effective flavonoid component derived from the medicinal plant Oroxylum indicum (L.) Kurz. In this study, the oleic acid (OA)-induced A549 cell injury model was established in vitro to explore the protective mechanism of Oroxin A. The experiment was divided into the following groups: control, OA and OA + Oroxin A group. The OA-induced A549 cell injury was dose (time)-dependent and was detected by the CCK-8 assay. The protein and mRNA expression levels associated with pyroptosis are detected by Western blotting and RT-qPCR. After Oroxin A treatment, the levels of IL-1β, IL-18 and LDH released were significantly lower than the OA group. In terms of pyroptosis, Oroxin A can inhibit the expression of pyroptosis-related protein and mRNA. Significantly, the surfactant protein C (SPC) level in the OA + Oroxin A group was significantly higher than that in the OA group. The treatment with Oroxin A alleviates the OA-induced injury in the A549 cells, and its mechanism may be related to the inhibition of A549 cells pyroptosis and prevention of the degradation of the SPC.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000800601&tlng=enOroxin Aoleic acidsurfactant protein CpyroptosisARDS |
| spellingShingle | JIAN HUANG CHEN CHEN JIANQIN XIE KERONG ZHAI SHILIN WEI XINGDONG CHENG RONGZHI ZHANG Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant Anais da Academia Brasileira de Ciências Oroxin A oleic acid surfactant protein C pyroptosis ARDS |
| title | Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant |
| title_full | Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant |
| title_fullStr | Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant |
| title_full_unstemmed | Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant |
| title_short | Oroxin A ameliorates the oleic acid-induced A549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant |
| title_sort | oroxin a ameliorates the oleic acid induced a549 cell injury through the suppression of pyroptosis and degradation of alveolar surfactant |
| topic | Oroxin A oleic acid surfactant protein C pyroptosis ARDS |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000800601&tlng=en |
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