Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in <i>Escherichia coli</i> and <i>Pseudomonas</i>

Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in <i>Escherichia coli</i> and <i>Pseudomonas</i>

Single-stranded DNA-binding proteins (SSBs) are essential for all living organisms. Whether SSBs can repair DNA double-strand breaks (DSBs) and improve the efficiency of CRISPR/Cas9-mediated genome editing has not been determined. Here, based on a pCas/pTargetF system, we constructed pCas-SSB and pC...

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Bibliographic Details
Main Authors: Ran Chai, Qi Zhang, Jie Wu, Ziwen Shi, Yanan Li, Yuqian Gao, Yuancheng Qi, Liyou Qiu
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Microorganisms
Subjects:
single-stranded DNA-binding protein
DNA double-strand breaks
dsDNA repair
CRISPR/Cas9 genome editing
homologous recombination
nonhomologous end joining
Online Access:https://www.mdpi.com/2076-2607/11/4/850

Internet

https://www.mdpi.com/2076-2607/11/4/850

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