Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans

Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introd...

Full description

Bibliographic Details
Main Authors: Carlos G. Silva-García, Anne Lanjuin, Caroline Heintz, Sneha Dutta, Nicole M. Clark, William B. Mair
Format: Article
Language:English
Published: Oxford University Press 2019-07-01
Series:G3: Genes, Genomes, Genetics
Subjects:
C. elegans
methods
CRISPR
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.119.400314

Internet

http://g3journal.org/lookup/doi/10.1534/g3.119.400314

Similar Items