Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introd...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-07-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.119.400314 |
| _version_ | 1819100049956667392 |
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| author | Carlos G. Silva-García Anne Lanjuin Caroline Heintz Sneha Dutta Nicole M. Clark William B. Mair |
| author_facet | Carlos G. Silva-García Anne Lanjuin Caroline Heintz Sneha Dutta Nicole M. Clark William B. Mair |
| author_sort | Carlos G. Silva-García |
| collection | DOAJ |
| description | We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans. |
| first_indexed | 2024-12-22T00:56:36Z |
| format | Article |
| id | doaj.art-01b6160a30a14edd9b392a8265bba243 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-22T00:56:36Z |
| publishDate | 2019-07-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-01b6160a30a14edd9b392a8265bba2432022-12-21T18:44:18ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362019-07-01972195219810.1534/g3.119.40031414Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegansCarlos G. Silva-GarcíaAnne LanjuinCaroline HeintzSneha DuttaNicole M. ClarkWilliam B. MairWe have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans.http://g3journal.org/lookup/doi/10.1534/g3.119.400314C. elegansmethodsCRISPR |
| spellingShingle | Carlos G. Silva-García Anne Lanjuin Caroline Heintz Sneha Dutta Nicole M. Clark William B. Mair Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans G3: Genes, Genomes, Genetics C. elegans methods CRISPR |
| title | Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans |
| title_full | Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans |
| title_fullStr | Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans |
| title_full_unstemmed | Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans |
| title_short | Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans |
| title_sort | single copy knock in loci for defined gene expression in caenorhabditis elegans |
| topic | C. elegans methods CRISPR |
| url | http://g3journal.org/lookup/doi/10.1534/g3.119.400314 |
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