Expression and purification of phage T7 ejection proteins for cryo-EM analysis

Expression and purification of phage T7 ejection proteins for cryo-EM analysis

Summary: Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery. Here,...

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Bibliographic Details
Main Authors: Nicholas A. Swanson, Ravi K. Lokareddy, Fenglin Li, Chun-feng Hou, Mikhail Pavlenok, Michael Niederweis, Gino Cingolani
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Microbiology
Microscopy
Protein Biochemistry
Protein expression and purification
Structural Biology
Cryo-EM
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006663

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http://www.sciencedirect.com/science/article/pii/S2666166721006663

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