Expression and purification of phage T7 ejection proteins for cryo-EM analysis
Summary: Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery. Here,...
| Autors principals: | , , , , , , |
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| Format: | Article |
| Idioma: | English |
| Publicat: |
Elsevier
2021-12-01
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| Col·lecció: | STAR Protocols |
| Matèries: | |
| Accés en línia: | http://www.sciencedirect.com/science/article/pii/S2666166721006663 |