Screening of media for in vitro culture and germplasm conservation in vitro of Ophioglossum thermale Kom(温泉瓶尔小草离体培养及种质试管保存培养基的筛选)
以温泉瓶尔小草新生幼叶为外植体，应用均匀设计法筛选其最适宜的离体培养和种质试管保存各阶段培养基.结果表明，最适合愈伤组织诱导的培养基为WPM + ZT0.50 mg • L-1+2，4-D1.90 mg • L-1，诱导率为96.5%；芽苗分化的培养基为WPM+ZT1.70 mg • L-1 +NAA0.10 mg • L-1，分化率为99.0%；生根培养基为1/4MS+IAA0.05 mg • L-1+IBA0.03 mg •L-1，生根率达97.8%以上；试管保存培养基为WPM+KT1.10 mg • L-1 +根皮苷3.20 mg • L-1，通过24个月的保存，芽苗生长率仅在6.5%以...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2011-03-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2011.02.018 |