High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis
Abstract Background The aim of study was to observe the effect of increased lactate levels during high-intensity interval training (HIIT) on protein lactylation, identify the target protein, and investigate the regulatory effect of lactylation on the function of the protein. Methods C57B/L6 mice wer...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Biology |
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| Online Access: | https://doi.org/10.1186/s12915-023-01698-9 |
| _version_ | 1797451446526935040 |
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| author | Xuefei Chen Wenhua Huang Jingbo Zhang Yanjun Li Zheng Xing Lanlan Guo Hongfeng Jiang Jing Zhang |
| author_facet | Xuefei Chen Wenhua Huang Jingbo Zhang Yanjun Li Zheng Xing Lanlan Guo Hongfeng Jiang Jing Zhang |
| author_sort | Xuefei Chen |
| collection | DOAJ |
| description | Abstract Background The aim of study was to observe the effect of increased lactate levels during high-intensity interval training (HIIT) on protein lactylation, identify the target protein, and investigate the regulatory effect of lactylation on the function of the protein. Methods C57B/L6 mice were divided into 3 groups: the control group, HIIT group, and dichloroacetate injection + HIIT group (DCA + HIIT). The HIIT and DCA + HIIT groups underwent 8 weeks of HIIT treatment, and the DCA + HIIT group was injected DCA before HIIT treatment. The expression of lipid metabolism-related genes was determined. Protein lactylation in subcutaneous adipose tissue was identified and analyzed using 4D label-free lactylation quantitative proteomics and bioinformatics analyses. The fatty acid synthase (FASN) lactylation and activity was determined. Results HIIT had a significant effect on fat loss; this effect was weakened when lactate production was inhibited. HIIT significantly upregulated the protein lactylation while lactate inhibition downregulated in iWAT. FASN had the most modification sites. Lactate treatment increased FASN lactylation levels, inhibited FASN activity, and reduced palmitate and triglyceride synthesis in 3T3-L1 cells. Conclusions This investigation revealed that lactate produced by HIIT increased protein pan-lactylation levels in iWAT. FASN lactylation inhibited de novo lipogenesis, which may be an important mechanism in HIIT-induced fat loss. |
| first_indexed | 2024-03-09T14:54:46Z |
| format | Article |
| id | doaj.art-01ec12becfb94dfa85540462e60b6905 |
| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-03-09T14:54:46Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj.art-01ec12becfb94dfa85540462e60b69052023-11-26T14:14:42ZengBMCBMC Biology1741-70072023-09-0121111410.1186/s12915-023-01698-9High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesisXuefei Chen0Wenhua Huang1Jingbo Zhang2Yanjun Li3Zheng Xing4Lanlan Guo5Hongfeng Jiang6Jing Zhang7College of P.E. and Sports, Beijing Normal UniversityCollege of P.E. and Sports, Beijing Normal UniversityCollege of P.E. and Sports, Beijing Normal UniversityCollege of P.E. and Sports, Beijing Normal UniversityCollege of P.E. and Sports, Beijing Normal UniversityCollege of P.E. and Sports, Beijing Normal UniversityExperimental Research Center, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical UniversityCollege of P.E. and Sports, Beijing Normal UniversityAbstract Background The aim of study was to observe the effect of increased lactate levels during high-intensity interval training (HIIT) on protein lactylation, identify the target protein, and investigate the regulatory effect of lactylation on the function of the protein. Methods C57B/L6 mice were divided into 3 groups: the control group, HIIT group, and dichloroacetate injection + HIIT group (DCA + HIIT). The HIIT and DCA + HIIT groups underwent 8 weeks of HIIT treatment, and the DCA + HIIT group was injected DCA before HIIT treatment. The expression of lipid metabolism-related genes was determined. Protein lactylation in subcutaneous adipose tissue was identified and analyzed using 4D label-free lactylation quantitative proteomics and bioinformatics analyses. The fatty acid synthase (FASN) lactylation and activity was determined. Results HIIT had a significant effect on fat loss; this effect was weakened when lactate production was inhibited. HIIT significantly upregulated the protein lactylation while lactate inhibition downregulated in iWAT. FASN had the most modification sites. Lactate treatment increased FASN lactylation levels, inhibited FASN activity, and reduced palmitate and triglyceride synthesis in 3T3-L1 cells. Conclusions This investigation revealed that lactate produced by HIIT increased protein pan-lactylation levels in iWAT. FASN lactylation inhibited de novo lipogenesis, which may be an important mechanism in HIIT-induced fat loss.https://doi.org/10.1186/s12915-023-01698-9High-intensity interval trainingLactylationFatty acid synthaseFat loss |
| spellingShingle | Xuefei Chen Wenhua Huang Jingbo Zhang Yanjun Li Zheng Xing Lanlan Guo Hongfeng Jiang Jing Zhang High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis BMC Biology High-intensity interval training Lactylation Fatty acid synthase Fat loss |
| title | High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis |
| title_full | High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis |
| title_fullStr | High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis |
| title_full_unstemmed | High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis |
| title_short | High-intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis |
| title_sort | high intensity interval training induces lactylation of fatty acid synthase to inhibit lipid synthesis |
| topic | High-intensity interval training Lactylation Fatty acid synthase Fat loss |
| url | https://doi.org/10.1186/s12915-023-01698-9 |
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