Protocol for determining protein dynamics using FT-IR spectroscopy

Summary: In biological systems, protein function depends on spatial and temporal changes known as protein dynamics, which can be probed by amide hydrogen/deuterium (H/D) exchange. Here, we present a protocol for determining protein dynamics by Fourier-transform infrared (FT-IR) spectroscopy. We desc...

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Bibliographic Details
Main Authors: Hao Shen, Cuiping Fu, Junting Zhang, Bin Feng, Shaoning Yu
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005543