Performance upgrade of a microbial explosives’ sensor strain by screening a high throughput saturation library of a transcriptional regulator

Performance upgrade of a microbial explosives’ sensor strain by screening a high throughput saturation library of a transcriptional regulator

We present a methodology for a high-throughput screening (HTS) of transcription factor libraries, based on bacterial cells and GFP fluorescence. The method is demonstrated on the Escherichia coli LysR-type transcriptional regulator YhaJ, a key element in 2,4-dinitrotuluene (DNT) detection by bacteri...

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Bibliographic Details
Main Authors: Lidor David, Etai Shpigel, Itay Levin, Shaked Moshe, Lior Zimmerman, Shilat Dadon-Simanowitz, Benjamin Shemer, Shon A. Levkovich, Liraz Larush, Shlomo Magdassi, Shimshon Belkin
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
Bioreporters
Bioluminescence
Escherichia coli
High-throughput screen (HTS)
Landmines
NGS
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037023002921

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http://www.sciencedirect.com/science/article/pii/S2001037023002921

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