Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts
A large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) regio...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-07-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714004495 |
| _version_ | 1819067670048276480 |
|---|---|
| author | Yosuke Tanaka Veronica Sanchez Nozomu Takata Tomomasa Yokomizo Yojiro Yamanaka Hiroshi Kataoka Philipp S. Hoppe Timm Schroeder Shin-Ichi Nishikawa |
| author_facet | Yosuke Tanaka Veronica Sanchez Nozomu Takata Tomomasa Yokomizo Yojiro Yamanaka Hiroshi Kataoka Philipp S. Hoppe Timm Schroeder Shin-Ichi Nishikawa |
| author_sort | Yosuke Tanaka |
| collection | DOAJ |
| description | A large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1+Gata1− population. A subset of this population moves from the extra- to intraembryonic region during E7.5–E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1+ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium. |
| first_indexed | 2024-12-21T16:21:56Z |
| format | Article |
| id | doaj.art-0217ad7dcae24351864d304bf1d950f8 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T16:21:56Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-0217ad7dcae24351864d304bf1d950f82022-12-21T18:57:32ZengElsevierCell Reports2211-12472014-07-0181313910.1016/j.celrep.2014.05.055Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic AngioblastsYosuke Tanaka0Veronica Sanchez1Nozomu Takata2Tomomasa Yokomizo3Yojiro Yamanaka4Hiroshi Kataoka5Philipp S. Hoppe6Timm Schroeder7Shin-Ichi Nishikawa8Laboratory of Stem Cell Biology, Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, JapanRosalind and Morris Goodman Cancer Research Centre, Department of Human Genetics, McGill University, Montreal, QC H3A 1A3, CanadaOrganogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Hematology, Juntendo University School of Medicine, 2-1-2 Hongo, Bunkyo-ku, Tokyo 113-8421, JapanRosalind and Morris Goodman Cancer Research Centre, Department of Human Genetics, McGill University, Montreal, QC H3A 1A3, CanadaLaboratory of Stem Cell Biology, Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, SwitzerlandLaboratory of Stem Cell Biology, Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, JapanA large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1+Gata1− population. A subset of this population moves from the extra- to intraembryonic region during E7.5–E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1+ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium.http://www.sciencedirect.com/science/article/pii/S2211124714004495 |
| spellingShingle | Yosuke Tanaka Veronica Sanchez Nozomu Takata Tomomasa Yokomizo Yojiro Yamanaka Hiroshi Kataoka Philipp S. Hoppe Timm Schroeder Shin-Ichi Nishikawa Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts Cell Reports |
| title | Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts |
| title_full | Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts |
| title_fullStr | Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts |
| title_full_unstemmed | Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts |
| title_short | Circulation-Independent Differentiation Pathway from Extraembryonic Mesoderm toward Hematopoietic Stem Cells via Hemogenic Angioblasts |
| title_sort | circulation independent differentiation pathway from extraembryonic mesoderm toward hematopoietic stem cells via hemogenic angioblasts |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714004495 |
| work_keys_str_mv | AT yosuketanaka circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT veronicasanchez circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT nozomutakata circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT tomomasayokomizo circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT yojiroyamanaka circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT hiroshikataoka circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT philippshoppe circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT timmschroeder circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts AT shinichinishikawa circulationindependentdifferentiationpathwayfromextraembryonicmesodermtowardhematopoieticstemcellsviahemogenicangioblasts |