Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention
Somatic human cells can divide a finite number of times, a phenomenon known as the Hayflick limit. It is based on the progressive erosion of the telomeric ends each time the cell completes a replicative cycle. Given this problem, researchers need cell lines that do not enter the senescence phase aft...
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MDPI AG
2023-01-01
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| Series: | BioTech |
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| Online Access: | https://www.mdpi.com/2673-6284/12/1/14 |
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| author | Javier Curi de Bardet Celeste Ramírez Cardentey Belkis López González Deanira Patrone Idania Lores Mulet Dario Siniscalco María de los Angeles Robinson-Agramonte |
| author_facet | Javier Curi de Bardet Celeste Ramírez Cardentey Belkis López González Deanira Patrone Idania Lores Mulet Dario Siniscalco María de los Angeles Robinson-Agramonte |
| author_sort | Javier Curi de Bardet |
| collection | DOAJ |
| description | Somatic human cells can divide a finite number of times, a phenomenon known as the Hayflick limit. It is based on the progressive erosion of the telomeric ends each time the cell completes a replicative cycle. Given this problem, researchers need cell lines that do not enter the senescence phase after a certain number of divisions. In this way, more lasting studies can be carried out over time and avoid the tedious work involved in performing cell passes to fresh media. However, some cells have a high replicative potential, such as embryonic stem cells and cancer cells. To accomplish this, these cells express the enzyme telomerase or activate the mechanisms of alternative telomere elongation, which favors the maintenance of the length of their stable telomeres. Researchers have been able to develop cell immortalization technology by studying the cellular and molecular bases of both mechanisms and the genes involved in the control of the cell cycle. Through it, cells with infinite replicative capacity are obtained. To obtain them, viral oncogenes/oncoproteins, myc genes, ectopic expression of telomerase, and the manipulation of genes that regulate the cell cycle, such as p53 and Rb, have been used. |
| first_indexed | 2024-03-11T06:51:12Z |
| format | Article |
| id | doaj.art-021b3bc83a2e4709907ddfe2f6c28a75 |
| institution | Directory Open Access Journal |
| issn | 2673-6284 |
| language | English |
| last_indexed | 2024-03-11T06:51:12Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | BioTech |
| spelling | doaj.art-021b3bc83a2e4709907ddfe2f6c28a752023-11-17T09:55:12ZengMDPI AGBioTech2673-62842023-01-011211410.3390/biotech12010014Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for ObtentionJavier Curi de Bardet0Celeste Ramírez Cardentey1Belkis López González2Deanira Patrone3Idania Lores Mulet4Dario Siniscalco5María de los Angeles Robinson-Agramonte6Department of Neurobiology, International Center for Neurological Restoration, Havana 11300, CubaDepartment of Virology, Tropical Medicine Institute Pedro Kouri, Havana 11400, CubaDepartment of Allergy, Calixto Garcia General University Hospital, Havana 10400, CubaDepartment of Experimental Medicine, Division of Molecular Biology, Biotechnology and Histology, University of Campania, 80138 Naples, ItalyRamon Gonzalez Coro Hospital, Havana 10400, CubaDepartment of Experimental Medicine, Division of Molecular Biology, Biotechnology and Histology, University of Campania, 80138 Naples, ItalyDepartment of Immunochemical, International Center for Neurological Restoration, Havana 11300, CubaSomatic human cells can divide a finite number of times, a phenomenon known as the Hayflick limit. It is based on the progressive erosion of the telomeric ends each time the cell completes a replicative cycle. Given this problem, researchers need cell lines that do not enter the senescence phase after a certain number of divisions. In this way, more lasting studies can be carried out over time and avoid the tedious work involved in performing cell passes to fresh media. However, some cells have a high replicative potential, such as embryonic stem cells and cancer cells. To accomplish this, these cells express the enzyme telomerase or activate the mechanisms of alternative telomere elongation, which favors the maintenance of the length of their stable telomeres. Researchers have been able to develop cell immortalization technology by studying the cellular and molecular bases of both mechanisms and the genes involved in the control of the cell cycle. Through it, cells with infinite replicative capacity are obtained. To obtain them, viral oncogenes/oncoproteins, myc genes, ectopic expression of telomerase, and the manipulation of genes that regulate the cell cycle, such as p53 and Rb, have been used.https://www.mdpi.com/2673-6284/12/1/14Hayflick limittelomerestelomerasealternative telomere elongationimmortalization |
| spellingShingle | Javier Curi de Bardet Celeste Ramírez Cardentey Belkis López González Deanira Patrone Idania Lores Mulet Dario Siniscalco María de los Angeles Robinson-Agramonte Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention BioTech Hayflick limit telomeres telomerase alternative telomere elongation immortalization |
| title | Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention |
| title_full | Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention |
| title_fullStr | Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention |
| title_full_unstemmed | Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention |
| title_short | Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention |
| title_sort | cell immortalization in vivo molecular bases and in vitro techniques for obtention |
| topic | Hayflick limit telomeres telomerase alternative telomere elongation immortalization |
| url | https://www.mdpi.com/2673-6284/12/1/14 |
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