Apolipoprotein E genotyping using the polymerase chain reaction and allele-specific oligonucleotide primers.

A method for apolipoprotein (apo) E genotyping was developed using the polymerase chain reaction (PCR) with allele-specific oligonucleotide primers (ASP). Synthetic oligonucleotides with base-pair mismatches at the 3' terminus were used as primers to amplify the apoE gene in subjects previously...

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Bibliographic Details
Main Authors: BF Main, PJ Jones, RT MacGillivray, DK Banfield
Format: Article
Language:English
Published: Elsevier 1991-01-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520422576