An Improved Method for Quantification of Viable <i>Fusarium</i> Cells in Infected Soil Products by Propidium Monoazide Coupled with Real-Time PCR
<i>Fusarium</i> is a soil-borne pathogen that causes root rot disease in cucumber. To date, quantitative real-time PCR (qPCR) is a common tool to detect the content of <i>Fusarium</i> in soil. However, qPCR cannot distinguish between viable and nonviable cells. The aim of thi...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-05-01
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Series: | Microorganisms |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2607/10/5/1037 |