An Improved Method for Quantification of Viable <i>Fusarium</i> Cells in Infected Soil Products by Propidium Monoazide Coupled with Real-Time PCR

An Improved Method for Quantification of Viable <i>Fusarium</i> Cells in Infected Soil Products by Propidium Monoazide Coupled with Real-Time PCR

<i>Fusarium</i> is a soil-borne pathogen that causes root rot disease in cucumber. To date, quantitative real-time PCR (qPCR) is a common tool to detect the content of <i>Fusarium</i> in soil. However, qPCR cannot distinguish between viable and nonviable cells. The aim of thi...

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Bibliographic Details
Main Authors: Lida Chen, Lei Li, Xuewen Xie, Ali Chai, Yanxia Shi, Tengfei Fan, Jianming Xie, Baoju Li
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Microorganisms
Subjects:
<i>Fusarium</i>
cucumber
propidium monoazide (PMA)
real-time PCR
viable cells
Online Access:https://www.mdpi.com/2076-2607/10/5/1037

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