Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo
Parkinson’s disease is considered to be due to an increase in the catabolism of dopamine by the action of monoamine oxidase (MAO) enzymes which leads to an increase in reactive oxygen species (ROS) and loss of dopaminergic neurons. Here, in a model of neurotoxicity inducible by 1-methyl-4-phenylpyri...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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IMR Press
2020-01-01
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| Series: | Frontiers in Bioscience-Scholar |
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| Online Access: | https://www.imrpress.com/journal/FBS/12/1/10.2741/S538 |
| _version_ | 1828789815289577472 |
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| author | Gabriela A. Perez-Barron Sergio Montes Moises Rubio-Osornio Jose G. Avila-Acevedo Sara Garcia-Jimenez Luis C. Rios Antonio Monroy-Noyola |
| author_facet | Gabriela A. Perez-Barron Sergio Montes Moises Rubio-Osornio Jose G. Avila-Acevedo Sara Garcia-Jimenez Luis C. Rios Antonio Monroy-Noyola |
| author_sort | Gabriela A. Perez-Barron |
| collection | DOAJ |
| description | Parkinson’s disease is considered to be due to an increase in the catabolism of dopamine by the action of monoamine oxidase (MAO) enzymes which leads to an increase in reactive oxygen species (ROS) and loss of dopaminergic neurons. Here, in a model of neurotoxicity inducible by 1-methyl-4-phenylpyridinium (MPP+), we tested the effect of hydroxytyrosol (HTy), a potent antioxidant, on generation of ROS. Five minutes after a single intravenous administration of 1.5 mg/Kg of Hty, Wistar rats received an intrastriatal micro-injection of 10 micrograms of MPP+ while control animals received saline solution. Six days later, all animals were treated with apomorphine (1 mg/Kg), subcutaneously and ipsilateral rotations were assessed within an hour. Then, the rats were sacrificed, striatal tissues were removed and their catecholamines and MAO-A and B activities were quantitated. Pretreatment with HTy significantly diminished the number of ipsilateral rotations. This recovery correlated with significant preservation of striatal dopamine and significant inhibition of of the MAO activity. These results are consistent with the inhibitory effect of HTy on the MAO isoforms and form a basis for the neuroprotective mechanism of this phenylpropanoid in MPP+ induced Parkinson’s disease. |
| first_indexed | 2024-12-12T01:19:29Z |
| format | Article |
| id | doaj.art-022e1f9366b74fd5a30600bd606b9fe2 |
| institution | Directory Open Access Journal |
| issn | 1945-0516 |
| language | English |
| last_indexed | 2024-12-12T01:19:29Z |
| publishDate | 2020-01-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Scholar |
| spelling | doaj.art-022e1f9366b74fd5a30600bd606b9fe22022-12-22T00:43:16ZengIMR PressFrontiers in Bioscience-Scholar1945-05162020-01-01121253710.2741/S538FBS-12-25Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivoGabriela A. Perez-Barron0Sergio Montes1Moises Rubio-Osornio2Jose G. Avila-Acevedo3Sara Garcia-Jimenez4Luis C. Rios5Antonio Monroy-Noyola6Laboratorio de Neuroproteccion, Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, MexicoDepartamento de Neuroquimica, Instituto Nacional de Neurologia y Neurocirugia, M.V.S., Av. Insurgentes Sur 3877, La Fama, Ciudad de Mexico, MexicoLaboratorio de Enfermedades Neurodegenerativas, Instituto Nacional de Neurologia y Neurocirugia, M.V.S., Av. Insurgentes Sur 3877, La Fama, Ciudad de Mexico, MexicoLaboratorio de Fitoquimica, UBIPRO, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autonoma de Mexico, Av. De los Barrios 1, Col. Los Reyes Ixtacala, Tlanepantla, Edo. de Mexico, MexicoLaboratorio de Neuroproteccion, Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, MexicoDepartamento de Neuroquimica, Instituto Nacional de Neurologia y Neurocirugia, M.V.S., Av. Insurgentes Sur 3877, La Fama, Ciudad de Mexico, MexicoLaboratorio de Neuroproteccion, Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, MexicoParkinson’s disease is considered to be due to an increase in the catabolism of dopamine by the action of monoamine oxidase (MAO) enzymes which leads to an increase in reactive oxygen species (ROS) and loss of dopaminergic neurons. Here, in a model of neurotoxicity inducible by 1-methyl-4-phenylpyridinium (MPP+), we tested the effect of hydroxytyrosol (HTy), a potent antioxidant, on generation of ROS. Five minutes after a single intravenous administration of 1.5 mg/Kg of Hty, Wistar rats received an intrastriatal micro-injection of 10 micrograms of MPP+ while control animals received saline solution. Six days later, all animals were treated with apomorphine (1 mg/Kg), subcutaneously and ipsilateral rotations were assessed within an hour. Then, the rats were sacrificed, striatal tissues were removed and their catecholamines and MAO-A and B activities were quantitated. Pretreatment with HTy significantly diminished the number of ipsilateral rotations. This recovery correlated with significant preservation of striatal dopamine and significant inhibition of of the MAO activity. These results are consistent with the inhibitory effect of HTy on the MAO isoforms and form a basis for the neuroprotective mechanism of this phenylpropanoid in MPP+ induced Parkinson’s disease.https://www.imrpress.com/journal/FBS/12/1/10.2741/S538hydroxytyrosolmpp+parkinson's diseasemonoamine oxidaseneuroprotection |
| spellingShingle | Gabriela A. Perez-Barron Sergio Montes Moises Rubio-Osornio Jose G. Avila-Acevedo Sara Garcia-Jimenez Luis C. Rios Antonio Monroy-Noyola Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo Frontiers in Bioscience-Scholar hydroxytyrosol mpp+ parkinson's disease monoamine oxidase neuroprotection |
| title | Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo |
| title_full | Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo |
| title_fullStr | Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo |
| title_full_unstemmed | Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo |
| title_short | Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP+ in vivo |
| title_sort | hydroxytyrosol inhibits mao isoforms and prevents neurotoxicity inducible by mpp in vivo |
| topic | hydroxytyrosol mpp+ parkinson's disease monoamine oxidase neuroprotection |
| url | https://www.imrpress.com/journal/FBS/12/1/10.2741/S538 |
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