Randomly barcoded transposon mutant libraries for gut commensals I: Strategies for efficient library construction

Summary: Randomly barcoded transposon mutant libraries are powerful tools for studying gene function and organization, assessing gene essentiality and pathways, discovering potential therapeutic targets, and understanding the physiology of gut bacteria and their interactions with the host. However,...

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Bibliographic Details
Main Authors: Surya Tripathi, Carlos Geert Pieter Voogdt, Stefan Oliver Bassler, Mary Anderson, Po-Hsun Huang, Nazgul Sakenova, Tümay Capraz, Sunit Jain, Alexandra Koumoutsi, Afonso Martins Bravo, Valentine Trotter, Michael Zimmerman, Justin L. Sonnenburg, Cullen Buie, Athanasios Typas, Adam M. Deutschbauer, Anthony L. Shiver, Kerwyn Casey Huang
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Cell Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124723015292