A contamination focused approach for optimizing the single-cell RNA-seq experiment

Summary: Droplet-based single-cell RNA-seq (scRNA-seq) data are plagued by ambient contaminations caused by nucleic acid material released by dead and dying cells. This material is mixed into the buffer and is co-encapsulated with cells, leading to a lower signal-to-noise ratio. Although there exist...

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Bibliographic Details
Main Authors:	Deronisha Arceneaux, Zhengyi Chen, Alan J. Simmons, Cody N. Heiser, Austin N. Southard-Smith, Michael J. Brenan, Yilin Yang, Bob Chen, Yanwen Xu, Eunyoung Choi, Joshua D. Campbell, Qi Liu, Ken S. Lau
Format:	Article
Language:	English
Published:	Elsevier 2023-07-01
Series:	iScience
Subjects:	Computational bioinformatics Transcriptomics Biology experimental methods
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004223013196

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http://www.sciencedirect.com/science/article/pii/S2589004223013196

A contamination focused approach for optimizing the single-cell RNA-seq experiment

Internet

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