Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.)
Chromium toxicity is considered as a major problem for agricultural soil that reduced crop productivity by affecting photosynthetic tissues. Exogenous application of melatonin can alleviate the adverse effects of chromium toxicity on plant growth. However, little is known about its effect on thylako...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020312081 |
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| author | Ahsan Ayyaz Misbah Amir Sarah Umer Muhammad Iqbal Hussan Bano Hafiza Saima Gul Yamna Noor Aneela kanwal Ayesha khalid Muhammad Javed Habib R. Athar Zafar Ullah Zafar Muhammad Ahsan Farooq |
| author_facet | Ahsan Ayyaz Misbah Amir Sarah Umer Muhammad Iqbal Hussan Bano Hafiza Saima Gul Yamna Noor Aneela kanwal Ayesha khalid Muhammad Javed Habib R. Athar Zafar Ullah Zafar Muhammad Ahsan Farooq |
| author_sort | Ahsan Ayyaz |
| collection | DOAJ |
| description | Chromium toxicity is considered as a major problem for agricultural soil that reduced crop productivity by affecting photosynthetic tissues. Exogenous application of melatonin can alleviate the adverse effects of chromium toxicity on plant growth. However, little is known about its effect on thylakoidal protein complexes responsible for conversion of solar energy to biochemical energy. Chlorophyll fluorescence a transients considered one of the best non-invasive and rapid method for the evaluation of photosynthetic (Photosystem II) efficiency of plants and plant health under environmental stress conditions. In the present study, three-week old plants of two canola cultivars AC-Excel and DGL were applied to melatonin (0, 1, 5, 10 μM) when grown under chromium stress (0, 50 and 100 μM) for further two weeks. Chromium stress reduced the growth (fresh and dry weights of shoots and roots) of both canola cultivars and exogenous application of 5 and 10 μM melatonin improved the growth of canola at 50 or 100 μM chromium stress. This improvement was greater in cv DGL than in AC-Excel. Increasing chromium decreased the photosynthetic pigments (chlorophyll a and chlorophyll b). However, 5 and 10 μM melatonin application improved chlorophyll a at 50 μM chromium stress. Structural stability and efficiency of photosystem II (PSII) measured as performance index (PIABS) and ratios of fluorescence (Fv/Fm, Fv/Fo) Fv decreased due to chromium stress. JIP-test parameters showed that chromium stress increased the absorption and trapping fluxes with decrease in electron transport fluxes which caused the damage to reaction centers (RC), detachment of oxygen evolving complex (OEC) from RC or inefficiency of electron transfer from OEC to RC. Such adverse effects were greater in cv AC-Excel. However exogenous application of melatonin improved PIABS, electron transport per reaction center (ET/RC), reduced variable fluorescence at J step (VJ) reflecting melatonin protected PSII from chromium stress induced damage by protecting OEC. Thus, OJIP fluorescence transients are quite helpful for understanding the intersystem electron transport beyond photosystem II in canola cultivars due to melatonin application under chromium stress. Findings: Exogenous application of melatonin alleviated toxic effects of chromium on plant growth of canola by modulating photosynthesis, enhanced photosystem II efficiency and regulation of electron transport flux to protect photo-inhibition of PSII from oxidative damage. |
| first_indexed | 2024-12-12T18:56:11Z |
| format | Article |
| id | doaj.art-0240ead1018d4ceda5156a105481c0fa |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-12T18:56:11Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0240ead1018d4ceda5156a105481c0fa2022-12-22T00:15:15ZengElsevierHeliyon2405-84402020-07-0167e04364Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.)Ahsan Ayyaz0Misbah Amir1Sarah Umer2Muhammad Iqbal3Hussan Bano4Hafiza Saima Gul5Yamna Noor6Aneela kanwal7Ayesha khalid8Muhammad Javed9Habib R. Athar10Zafar Ullah Zafar11Muhammad Ahsan Farooq12Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, Pakistan; Corresponding author.Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, Pakistan; Department of Botany, University of Okara, 56300, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, Pakistan; Department of Botany, The Women University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, Pakistan; Department of Botany, The Women University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, Pakistan; Department of Botany, University of Education, Sub Campus Dera Ghazi Khan, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanInstitute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60000, PakistanChromium toxicity is considered as a major problem for agricultural soil that reduced crop productivity by affecting photosynthetic tissues. Exogenous application of melatonin can alleviate the adverse effects of chromium toxicity on plant growth. However, little is known about its effect on thylakoidal protein complexes responsible for conversion of solar energy to biochemical energy. Chlorophyll fluorescence a transients considered one of the best non-invasive and rapid method for the evaluation of photosynthetic (Photosystem II) efficiency of plants and plant health under environmental stress conditions. In the present study, three-week old plants of two canola cultivars AC-Excel and DGL were applied to melatonin (0, 1, 5, 10 μM) when grown under chromium stress (0, 50 and 100 μM) for further two weeks. Chromium stress reduced the growth (fresh and dry weights of shoots and roots) of both canola cultivars and exogenous application of 5 and 10 μM melatonin improved the growth of canola at 50 or 100 μM chromium stress. This improvement was greater in cv DGL than in AC-Excel. Increasing chromium decreased the photosynthetic pigments (chlorophyll a and chlorophyll b). However, 5 and 10 μM melatonin application improved chlorophyll a at 50 μM chromium stress. Structural stability and efficiency of photosystem II (PSII) measured as performance index (PIABS) and ratios of fluorescence (Fv/Fm, Fv/Fo) Fv decreased due to chromium stress. JIP-test parameters showed that chromium stress increased the absorption and trapping fluxes with decrease in electron transport fluxes which caused the damage to reaction centers (RC), detachment of oxygen evolving complex (OEC) from RC or inefficiency of electron transfer from OEC to RC. Such adverse effects were greater in cv AC-Excel. However exogenous application of melatonin improved PIABS, electron transport per reaction center (ET/RC), reduced variable fluorescence at J step (VJ) reflecting melatonin protected PSII from chromium stress induced damage by protecting OEC. Thus, OJIP fluorescence transients are quite helpful for understanding the intersystem electron transport beyond photosystem II in canola cultivars due to melatonin application under chromium stress. Findings: Exogenous application of melatonin alleviated toxic effects of chromium on plant growth of canola by modulating photosynthesis, enhanced photosystem II efficiency and regulation of electron transport flux to protect photo-inhibition of PSII from oxidative damage.http://www.sciencedirect.com/science/article/pii/S2405844020312081OJIPChlorophyll fluorescenceMelatoninChromium stressCanolaFood science |
| spellingShingle | Ahsan Ayyaz Misbah Amir Sarah Umer Muhammad Iqbal Hussan Bano Hafiza Saima Gul Yamna Noor Aneela kanwal Ayesha khalid Muhammad Javed Habib R. Athar Zafar Ullah Zafar Muhammad Ahsan Farooq Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.) Heliyon OJIP Chlorophyll fluorescence Melatonin Chromium stress Canola Food science |
| title | Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.) |
| title_full | Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.) |
| title_fullStr | Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.) |
| title_full_unstemmed | Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.) |
| title_short | Melatonin induced changes in photosynthetic efficiency as probed by OJIP associated with improved chromium stress tolerance in canola (Brassica napus L.) |
| title_sort | melatonin induced changes in photosynthetic efficiency as probed by ojip associated with improved chromium stress tolerance in canola brassica napus l |
| topic | OJIP Chlorophyll fluorescence Melatonin Chromium stress Canola Food science |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020312081 |
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