Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells
The senescence of cardiovascular endothelial cells (ECs) is a major risk factor in the development of aging-related cardiovascular diseases. However, the molecular dynamics in cardiovascular EC aging are poorly understood. Here, we characterized the transcriptomic landscape of cardiovascular ECs dur...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.900978/full |
| _version_ | 1817982622384521216 |
|---|---|
| author | Bo Gou Bo Gou Bo Gou Bo Gou Xiaojing Chu Xiaojing Chu Xiaojing Chu Yi Xiao Yi Xiao Yi Xiao Yi Xiao Pinxuan Liu Pinxuan Liu Pinxuan Liu Pinxuan Liu Hao Zhang Hao Zhang Hao Zhang Hao Zhang Zeyu Gao Zeyu Gao Zeyu Gao Moshi Song Moshi Song Moshi Song Moshi Song |
| author_facet | Bo Gou Bo Gou Bo Gou Bo Gou Xiaojing Chu Xiaojing Chu Xiaojing Chu Yi Xiao Yi Xiao Yi Xiao Yi Xiao Pinxuan Liu Pinxuan Liu Pinxuan Liu Pinxuan Liu Hao Zhang Hao Zhang Hao Zhang Hao Zhang Zeyu Gao Zeyu Gao Zeyu Gao Moshi Song Moshi Song Moshi Song Moshi Song |
| author_sort | Bo Gou |
| collection | DOAJ |
| description | The senescence of cardiovascular endothelial cells (ECs) is a major risk factor in the development of aging-related cardiovascular diseases. However, the molecular dynamics in cardiovascular EC aging are poorly understood. Here, we characterized the transcriptomic landscape of cardiovascular ECs during aging and observed that ribosome biogenesis, inflammation, apoptosis and angiogenesis-related genes and pathways changed with age. We also highlighted the importance of collagen genes in the crosstalk between ECs and other cell types in cardiovascular aging. Moreover, transcriptional regulatory network analysis revealed Jun as a candidate transcription factor involved in murine cardiovascular senescence and we validated the upregulation of Jun in aged cardiovascular ECs both in vitro and in vivo. Altogether, our study reveals the transcriptomic reprogramming in the aging murine cardiovascular ECs, which deepens the understanding of the molecular mechanisms of cardiovascular aging and provides new insights into potential therapeutic targets against age-related cardiovascular diseases. |
| first_indexed | 2024-04-13T23:23:01Z |
| format | Article |
| id | doaj.art-02467fd014e24365aac727a45c509219 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-13T23:23:01Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-02467fd014e24365aac727a45c5092192022-12-22T02:25:10ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-05-01910.3389/fcvm.2022.900978900978Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial CellsBo Gou0Bo Gou1Bo Gou2Bo Gou3Xiaojing Chu4Xiaojing Chu5Xiaojing Chu6Yi Xiao7Yi Xiao8Yi Xiao9Yi Xiao10Pinxuan Liu11Pinxuan Liu12Pinxuan Liu13Pinxuan Liu14Hao Zhang15Hao Zhang16Hao Zhang17Hao Zhang18Zeyu Gao19Zeyu Gao20Zeyu Gao21Moshi Song22Moshi Song23Moshi Song24Moshi Song25State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaBeijing Institute for Stem Cell and Regenerative Medicine, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe senescence of cardiovascular endothelial cells (ECs) is a major risk factor in the development of aging-related cardiovascular diseases. However, the molecular dynamics in cardiovascular EC aging are poorly understood. Here, we characterized the transcriptomic landscape of cardiovascular ECs during aging and observed that ribosome biogenesis, inflammation, apoptosis and angiogenesis-related genes and pathways changed with age. We also highlighted the importance of collagen genes in the crosstalk between ECs and other cell types in cardiovascular aging. Moreover, transcriptional regulatory network analysis revealed Jun as a candidate transcription factor involved in murine cardiovascular senescence and we validated the upregulation of Jun in aged cardiovascular ECs both in vitro and in vivo. Altogether, our study reveals the transcriptomic reprogramming in the aging murine cardiovascular ECs, which deepens the understanding of the molecular mechanisms of cardiovascular aging and provides new insights into potential therapeutic targets against age-related cardiovascular diseases.https://www.frontiersin.org/articles/10.3389/fcvm.2022.900978/fullsenescencecardiovascular endothelial cellsscRNA-seqage-dependent genestranscriptomic reprogramming |
| spellingShingle | Bo Gou Bo Gou Bo Gou Bo Gou Xiaojing Chu Xiaojing Chu Xiaojing Chu Yi Xiao Yi Xiao Yi Xiao Yi Xiao Pinxuan Liu Pinxuan Liu Pinxuan Liu Pinxuan Liu Hao Zhang Hao Zhang Hao Zhang Hao Zhang Zeyu Gao Zeyu Gao Zeyu Gao Moshi Song Moshi Song Moshi Song Moshi Song Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells Frontiers in Cardiovascular Medicine senescence cardiovascular endothelial cells scRNA-seq age-dependent genes transcriptomic reprogramming |
| title | Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells |
| title_full | Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells |
| title_fullStr | Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells |
| title_full_unstemmed | Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells |
| title_short | Single-Cell Analysis Reveals Transcriptomic Reprogramming in Aging Cardiovascular Endothelial Cells |
| title_sort | single cell analysis reveals transcriptomic reprogramming in aging cardiovascular endothelial cells |
| topic | senescence cardiovascular endothelial cells scRNA-seq age-dependent genes transcriptomic reprogramming |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.900978/full |
| work_keys_str_mv | AT bogou singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT bogou singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT bogou singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT bogou singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT xiaojingchu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT xiaojingchu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT xiaojingchu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT yixiao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT yixiao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT yixiao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT yixiao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT pinxuanliu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT pinxuanliu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT pinxuanliu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT pinxuanliu singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT haozhang singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT haozhang singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT haozhang singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT haozhang singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT zeyugao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT zeyugao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT zeyugao singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT moshisong singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT moshisong singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT moshisong singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells AT moshisong singlecellanalysisrevealstranscriptomicreprogramminginagingcardiovascularendothelialcells |