Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Abstract NanoLuc, a superior β-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques are combined, revealing that imidazopyrazinone luciferins bind to an intra-barrel catalytic site but also to an alloster...

Full description

Bibliographic Details
Main Authors: Michal Nemergut, Daniel Pluskal, Jana Horackova, Tereza Sustrova, Jan Tulis, Tomas Barta, Racha Baatallah, Glwadys Gagnot, Veronika Novakova, Marika Majerova, Karolina Sedlackova, Sérgio M. Marques, Martin Toul, Jiri Damborsky, Zbynek Prokop, David Bednar, Yves L. Janin, Martin Marek
Format: Article
Language:English
Published: Nature Portfolio 2023-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-43403-y

Internet

https://doi.org/10.1038/s41467-023-43403-y

Similar Items