Characterizing and Improving pET Vectors for Cell-free Expression

Cell-free protein synthesis (CFPS) is an in vitro process that enables diverse applications in research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using minimal laboratory equipment and reagents. One of the major limitations of CFPS implementation is its sensitivity to...

Full description

Bibliographic Details
Main Authors: Kara Jew, Philip E. J. Smith, Byungcheol So, Jillian Kasman, Javin P. Oza, Michael W. Black
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.895069/full