New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventio...

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Bibliographic Details
Main Authors: Nathan Wales, Christian Carøe, Marcela Sandoval-Velasco, Cristina Gamba, Ross Barnett, José Alfredo Samaniego, Jazmín Ramos Madrigal, Ludovic Orlando, M. Thomas P. Gilbert
Format: Article
Language:English
Published: Taylor & Francis Group 2015-12-01
Series:BioTechniques
Subjects:
ancient DNA
paleogenomics
DNA library preparation
next-generation sequencing
Online Access:https://www.future-science.com/doi/10.2144/000114364

Internet

https://www.future-science.com/doi/10.2144/000114364

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