Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling
Methods to identify protein interaction networks often suffer from poor spatiotemporal resolution. Here the authors present a light-activated proximity labeling method where the protein of interest is fused to the photosensitizer protein miniSOG, allowing temporally resolved labeling of interactors.
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32689-z |
| _version_ | 1811340519457947648 |
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| author | Yansheng Zhai Xiaoyan Huang Keren Zhang Yuchen Huang Yanlong Jiang Jingwei Cui Zhe Zhang Cookson K. C. Chiu Weiye Zhong Gang Li |
| author_facet | Yansheng Zhai Xiaoyan Huang Keren Zhang Yuchen Huang Yanlong Jiang Jingwei Cui Zhe Zhang Cookson K. C. Chiu Weiye Zhong Gang Li |
| author_sort | Yansheng Zhai |
| collection | DOAJ |
| description | Methods to identify protein interaction networks often suffer from poor spatiotemporal resolution. Here the authors present a light-activated proximity labeling method where the protein of interest is fused to the photosensitizer protein miniSOG, allowing temporally resolved labeling of interactors. |
| first_indexed | 2024-04-13T18:43:09Z |
| format | Article |
| id | doaj.art-02955cce232c437cb11554572b2a401a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T18:43:09Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-02955cce232c437cb11554572b2a401a2022-12-22T02:34:40ZengNature PortfolioNature Communications2041-17232022-08-0113111210.1038/s41467-022-32689-zSpatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labelingYansheng Zhai0Xiaoyan Huang1Keren Zhang2Yuchen Huang3Yanlong Jiang4Jingwei Cui5Zhe Zhang6Cookson K. C. Chiu7Weiye Zhong8Gang Li9Institute of Systems and Physical Biology, Shenzhen Bay LaboratoryInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryDepartment of Chemistry, UF Scripps Biomedical ResearchInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryMulti-omics Mass Spectrometry Core, Office of Core Facility, Shenzhen Bay LaboratoryInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryInstitute of Systems and Physical Biology, Shenzhen Bay LaboratoryMethods to identify protein interaction networks often suffer from poor spatiotemporal resolution. Here the authors present a light-activated proximity labeling method where the protein of interest is fused to the photosensitizer protein miniSOG, allowing temporally resolved labeling of interactors.https://doi.org/10.1038/s41467-022-32689-z |
| spellingShingle | Yansheng Zhai Xiaoyan Huang Keren Zhang Yuchen Huang Yanlong Jiang Jingwei Cui Zhe Zhang Cookson K. C. Chiu Weiye Zhong Gang Li Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling Nature Communications |
| title | Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling |
| title_full | Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling |
| title_fullStr | Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling |
| title_full_unstemmed | Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling |
| title_short | Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling |
| title_sort | spatiotemporal resolved protein networks profiling with photoactivation dependent proximity labeling |
| url | https://doi.org/10.1038/s41467-022-32689-z |
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