Efficient Cloning Method that Selects the Recombinant Clones
Directional cloning using cohesive ends is the most efficient cloning method. However, sometimes it is necessary to use blunt ends to clone a DNA fragment into the plasmid vector. Compared with that of cohesive ends, efficiency of blunt-end ligation is low. Compared with the native blunt ends (e.g.,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97232st05 |