Targeted rRNA depletion enables efficient mRNA sequencing in diverse bacterial species and complex co-cultures

Targeted rRNA depletion enables efficient mRNA sequencing in diverse bacterial species and complex co-cultures

ABSTRACTBacterial mRNA sequencing is inefficient due to the abundance of ribosomal RNA that is challenging to deplete. While commercial kits target rRNA from common bacterial species, they are frequently inefficient when applied to divergent species, including those from environmental isolates. Simi...

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Bibliographic Details
Main Authors: Kellie A. Heom, Chatarin Wangsanuwat, Lazarina V. Butkovich, Scott C. Tam, Annette R. Rowe, Michelle A. O'Malley, Siddharth S. Dey
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:mSystems
Subjects:
bacterial mRNA sequencing
rRNA depletion
non-model microbial sequencing
fungal and bacterial co-cultures
lignocellulose deconstruction
Online Access:https://journals.asm.org/doi/10.1128/msystems.00281-23

Internet

https://journals.asm.org/doi/10.1128/msystems.00281-23

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