In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase.
Under the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation. However, a comprehensive understanding of how this regulat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3461030?pdf=render |
| _version_ | 1819089432249106432 |
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| author | Yoke-Peng Lee Chi-Hang Wong Kheng-Sze Chan Soak-Kuan Lai Cheng-Gee Koh Hoi-Yeung Li |
| author_facet | Yoke-Peng Lee Chi-Hang Wong Kheng-Sze Chan Soak-Kuan Lai Cheng-Gee Koh Hoi-Yeung Li |
| author_sort | Yoke-Peng Lee |
| collection | DOAJ |
| description | Under the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation. However, a comprehensive understanding of how this regulation is mechanistically achieved remains elusive. Using our newly designed live cell FRET time-lapse imaging, we found that post-metaphase RanGTP is crucial in the maintenance of stable kinetochore-microtubule attachments by regulating Aurora B kinase via the NES-bearing Mst1. More importantly, our study demonstrates that by ensuring stable alignment of metaphase chromosomes prior to segregation, RanGTP is indispensible in governing the genomic integrity and the fidelity of cell cycle progression. Our findings suggest an additional role of RanGTP beyond its known function in mitotic spindle assembly during the prometaphase-metaphase transition. |
| first_indexed | 2024-12-21T22:07:50Z |
| format | Article |
| id | doaj.art-02d150a3740f4983ba217505023c2c71 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:07:50Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-02d150a3740f4983ba217505023c2c712022-12-21T18:48:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4583610.1371/journal.pone.0045836In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase.Yoke-Peng LeeChi-Hang WongKheng-Sze ChanSoak-Kuan LaiCheng-Gee KohHoi-Yeung LiUnder the fluctuating circumstances provided by the innate dynamics of microtubules and opposing tensions resulted from microtubule-associated motors, it is vital to ensure stable kinetochore-microtubule attachments for accurate segregation. However, a comprehensive understanding of how this regulation is mechanistically achieved remains elusive. Using our newly designed live cell FRET time-lapse imaging, we found that post-metaphase RanGTP is crucial in the maintenance of stable kinetochore-microtubule attachments by regulating Aurora B kinase via the NES-bearing Mst1. More importantly, our study demonstrates that by ensuring stable alignment of metaphase chromosomes prior to segregation, RanGTP is indispensible in governing the genomic integrity and the fidelity of cell cycle progression. Our findings suggest an additional role of RanGTP beyond its known function in mitotic spindle assembly during the prometaphase-metaphase transition.http://europepmc.org/articles/PMC3461030?pdf=render |
| spellingShingle | Yoke-Peng Lee Chi-Hang Wong Kheng-Sze Chan Soak-Kuan Lai Cheng-Gee Koh Hoi-Yeung Li In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. PLoS ONE |
| title | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. |
| title_full | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. |
| title_fullStr | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. |
| title_full_unstemmed | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. |
| title_short | In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase. |
| title_sort | in vivo fret imaging revealed a regulatory role of rangtp in kinetochore microtubule attachments via aurora b kinase |
| url | http://europepmc.org/articles/PMC3461030?pdf=render |
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