β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice
The medial prefrontal cortex (mPFC) and β-adrenoceptors (βARs) have been implicated in modulating anxiety-like behavior. However, the specific contributions of the β2-AR subtype in mPFC in anxiety are still unclear. To address this issue, we used optogenetic and microRNA-based (miRNA) silencing to d...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5578 |
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| author | Zhuogui Lei Yukyan Lam Cheukhin Li Zhongqi Fu Aruna S. Ramkrishnan Shu Liu Ying Li |
| author_facet | Zhuogui Lei Yukyan Lam Cheukhin Li Zhongqi Fu Aruna S. Ramkrishnan Shu Liu Ying Li |
| author_sort | Zhuogui Lei |
| collection | DOAJ |
| description | The medial prefrontal cortex (mPFC) and β-adrenoceptors (βARs) have been implicated in modulating anxiety-like behavior. However, the specific contributions of the β2-AR subtype in mPFC in anxiety are still unclear. To address this issue, we used optogenetic and microRNA-based (miRNA) silencing to dissect the role of β2-AR in mPFC in anxiety-like behavior. On the one hand, we use a chimeric rhodopsin/β2-AR (Opto-β2-AR) with in vivo optogenetic techniques to selectively activate β2-adrenergic signaling in excitatory neurons of the mPFC. We found that opto-activation of β2-AR is sufficient to induce anxiety-like behavior and reduce social interaction. On the other hand, we utilize the miRNA silencing technique to specifically knock down the β2-AR in mPFC excitatory neurons. We found that the β2-AR knock down induces anxiolytic-like behavior and promotes social interaction compared to the control group. These data suggest that β2-AR signaling in the mPFC has a critical role in anxiety-like states. These findings suggest that inhibiting of β2-AR signaling in the mPFC may be an effective treatment of anxiety disorders. |
| first_indexed | 2024-03-10T03:44:02Z |
| format | Article |
| id | doaj.art-02ecb872926d405eaf44a0709c4f7399 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:02Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-02ecb872926d405eaf44a0709c4f73992023-11-23T11:25:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310557810.3390/ijms23105578β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in MiceZhuogui Lei0Yukyan Lam1Cheukhin Li2Zhongqi Fu3Aruna S. Ramkrishnan4Shu Liu5Ying Li6Department of Neuroscience, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong 999077, ChinaThe medial prefrontal cortex (mPFC) and β-adrenoceptors (βARs) have been implicated in modulating anxiety-like behavior. However, the specific contributions of the β2-AR subtype in mPFC in anxiety are still unclear. To address this issue, we used optogenetic and microRNA-based (miRNA) silencing to dissect the role of β2-AR in mPFC in anxiety-like behavior. On the one hand, we use a chimeric rhodopsin/β2-AR (Opto-β2-AR) with in vivo optogenetic techniques to selectively activate β2-adrenergic signaling in excitatory neurons of the mPFC. We found that opto-activation of β2-AR is sufficient to induce anxiety-like behavior and reduce social interaction. On the other hand, we utilize the miRNA silencing technique to specifically knock down the β2-AR in mPFC excitatory neurons. We found that the β2-AR knock down induces anxiolytic-like behavior and promotes social interaction compared to the control group. These data suggest that β2-AR signaling in the mPFC has a critical role in anxiety-like states. These findings suggest that inhibiting of β2-AR signaling in the mPFC may be an effective treatment of anxiety disorders.https://www.mdpi.com/1422-0067/23/10/5578norepinephrineβ2-adrenoceptorsoptogeneticsneuronal manipulationmicroRNA-based silencingmedial prefrontal cortex |
| spellingShingle | Zhuogui Lei Yukyan Lam Cheukhin Li Zhongqi Fu Aruna S. Ramkrishnan Shu Liu Ying Li β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice International Journal of Molecular Sciences norepinephrine β2-adrenoceptors optogenetics neuronal manipulation microRNA-based silencing medial prefrontal cortex |
| title | β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice |
| title_full | β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice |
| title_fullStr | β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice |
| title_full_unstemmed | β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice |
| title_short | β2-Adrenoceptors in the Medial Prefrontal Cortex Excitatory Neurons Regulate Anxiety-like Behavior in Mice |
| title_sort | β2 adrenoceptors in the medial prefrontal cortex excitatory neurons regulate anxiety like behavior in mice |
| topic | norepinephrine β2-adrenoceptors optogenetics neuronal manipulation microRNA-based silencing medial prefrontal cortex |
| url | https://www.mdpi.com/1422-0067/23/10/5578 |
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