Co-transformation of bean callus using high-velocity microprojectiles- mediated DNA transfer

Co-transformation of bean callus using high-velocity microprojectiles- mediated DNA transfer

We have found that 50 mg/I kanamycin and 0.8 Mo1/1 mannitol concentration was sufficient to kill the control callus of bean (Phaseolus vulgaris L.) and differentiate transgenic from the non-transgenic cells. The GeneBooster particle delivery system was used for the bombardment of bean callus. The ka...

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Bibliographic Details
Main Authors: E. Eissa Ahmed, Gy. Bisztray, I. Velich
Format: Article
Language:English
Published: University of Debrecen 2001-03-01
Series:International Journal of Horticultural Science
Subjects:
Phaseolus vulgaris
callus
GeneBooster
kanamycin resistance
mannitol
transformation
Online Access:https://ojs.lib.unideb.hu/IJHS/article/view/253

Internet

https://ojs.lib.unideb.hu/IJHS/article/view/253

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