Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Summary: Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dyn...

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Bibliographic Details
Main Authors: Yanxiong Pan, Hui Li, Qiaobin Li, Mary Lenertz, Isabelle Schuster, Drew Jordahl, Xiao Zhu, Bingcan Chen, Zhongyu Yang
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Biophysics
Molecular/Chemical Probes
Protein Biochemistry
Protein expression and purification
Structural Biology
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100383X

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http://www.sciencedirect.com/science/article/pii/S266616672100383X

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