Generation of 2C-like mouse embryonic stem cells in vivo to evaluate developmental potency

Generation of 2C-like mouse embryonic stem cells in vivo to evaluate developmental potency

Summary: This protocol describes an optimized workflow for generating 2-cell (2C) stage-like mouse embryonic stem cells (mESCs) for microinjection into 8-cell stage mouse embryos to evaluate the developmental potency of these cells. We detail the following steps: 1) chemical suppression of glycolysi...

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Bibliographic Details
Main Authors: Dennis EK Tan, Baojiang Wu, Siqin Bao, Wee-Wei Tee
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Flow cytometry/Mass cytometry
Developmental biology
Microscopy
Stem cells
Cell differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005640

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http://www.sciencedirect.com/science/article/pii/S2666166722005640

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