Three-Dimensional Visualization of APEX2-Tagged Erg11 in <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Using Focused Ion Beam Scanning Electron Microscopy

Three-Dimensional Visualization of APEX2-Tagged Erg11 in <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Using Focused Ion Beam Scanning Electron Microscopy

ABSTRACT The determination of the exact location of a protein in the cell is essential to the understanding of biological processes. Here, we report for the first time the visualization of a protein of interest in Saccharomyces cerevisiae using focused ion beam scanning electron microscopy (FIB-SEM)...

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Bibliographic Details
Main Authors: Winnie Kerstens, Anneke Kremer, Michelle Holtappels, Peter Borghgraef, Saskia Lippens, Patrick Van Dijck
Format: Article
Language:English
Published: American Society for Microbiology 2020-02-01
Series:mSphere
Subjects:
FIB-SEM
electron microscopy
Saccharomyces cerevisiae
yeast
APEX2
DAB
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00981-19

Internet

https://journals.asm.org/doi/10.1128/mSphere.00981-19

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