Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility

Objectives Leucocytospermia is a rare cause of infertility with a variable incidence in infertile men. In many andrology laboratories, semen analysis is primarily centered on analyzing basic parameters (sperm count, motility, and viability). We examined the role of cytomorphological analysis on Papa...

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Main Authors: Vandana Puri, Kavita Gaur, Sunaina Hooda, Shailaja Shukla, Sunita Sharma
Format: Article
Language:English
Published: Thieme Medical and Scientific Publishers Pvt. Ltd. 2021-06-01
Series:Journal of Laboratory Physicians
Subjects:
Online Access:http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1730846
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author Vandana Puri
Kavita Gaur
Sunaina Hooda
Shailaja Shukla
Sunita Sharma
author_facet Vandana Puri
Kavita Gaur
Sunaina Hooda
Shailaja Shukla
Sunita Sharma
author_sort Vandana Puri
collection DOAJ
description Objectives Leucocytospermia is a rare cause of infertility with a variable incidence in infertile men. In many andrology laboratories, semen analysis is primarily centered on analyzing basic parameters (sperm count, motility, and viability). We examined the role of cytomorphological analysis on Papanicolaou (PAP)-stained smears in the work up of male infertility, with special reference to leukocytospermia and assessed the morphological features of sperms in these cases. Materials and Methods All cases signed out as “leukocytospermia” between November 2017 and January 2018 were evaluated. Cases showing pus cells (≥ 1/5hpf) on cytosmear evaluation were also analyzed. Parameters obtained on modified Neubauer’s chamber, wet mount preparations, and autoanalyzer SQA-IIC-P (Medical Electronic Systems, Israel) were tabulated. PAP-stained smears were examined to assess morphological defects and other findings, if any. Results Out of 348 semen samples, 6 (1.72%) were diagnosed as leukocytospermia, all displaying pus cells on cytomorphological evaluation. Five cases having white blood cells < 1 × 10 9/L were analyzed, which displayed the presence of pus cells (≥ 1/5 hpf) on cytosmear preparations (1.43%). Nine cases (81.8%) showed reduced motile sperm concentration, functional sperm concentration, and sperm motility index. Morphological evaluation of the PAP-stained smears showed mid-piece defects in 18.1% cases. Conclusion Diligent cytomorphological evaluation of semen is essential to diagnose leukocytospermia, assess morphological defects, and serve as a quality control measure.
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spelling doaj.art-0338389061eb4895b22ff80a14adf4ef2022-12-21T17:16:20ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262021-06-01130320821310.1055/s-0041-1730846Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of UtilityVandana Puri0Kavita Gaur1Sunaina Hooda2Shailaja Shukla3Sunita Sharma4Department of Pathology, Lady Hardinge Medical College, Shaheed Bhagat Singh Marg, New Delhi, IndiaDepartment of Pathology, Lady Hardinge Medical College, Shaheed Bhagat Singh Marg, New Delhi, IndiaDepartment of Pathology, Lady Hardinge Medical College, Shaheed Bhagat Singh Marg, New Delhi, IndiaDepartment of Pathology, Lady Hardinge Medical College, Shaheed Bhagat Singh Marg, New Delhi, IndiaDepartment of Pathology, Lady Hardinge Medical College, Shaheed Bhagat Singh Marg, New Delhi, IndiaObjectives Leucocytospermia is a rare cause of infertility with a variable incidence in infertile men. In many andrology laboratories, semen analysis is primarily centered on analyzing basic parameters (sperm count, motility, and viability). We examined the role of cytomorphological analysis on Papanicolaou (PAP)-stained smears in the work up of male infertility, with special reference to leukocytospermia and assessed the morphological features of sperms in these cases. Materials and Methods All cases signed out as “leukocytospermia” between November 2017 and January 2018 were evaluated. Cases showing pus cells (≥ 1/5hpf) on cytosmear evaluation were also analyzed. Parameters obtained on modified Neubauer’s chamber, wet mount preparations, and autoanalyzer SQA-IIC-P (Medical Electronic Systems, Israel) were tabulated. PAP-stained smears were examined to assess morphological defects and other findings, if any. Results Out of 348 semen samples, 6 (1.72%) were diagnosed as leukocytospermia, all displaying pus cells on cytomorphological evaluation. Five cases having white blood cells < 1 × 10 9/L were analyzed, which displayed the presence of pus cells (≥ 1/5 hpf) on cytosmear preparations (1.43%). Nine cases (81.8%) showed reduced motile sperm concentration, functional sperm concentration, and sperm motility index. Morphological evaluation of the PAP-stained smears showed mid-piece defects in 18.1% cases. Conclusion Diligent cytomorphological evaluation of semen is essential to diagnose leukocytospermia, assess morphological defects, and serve as a quality control measure.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1730846leucocytospermiainfertilityreactive oxygen speciessemen
spellingShingle Vandana Puri
Kavita Gaur
Sunaina Hooda
Shailaja Shukla
Sunita Sharma
Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility
Journal of Laboratory Physicians
leucocytospermia
infertility
reactive oxygen species
semen
title Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility
title_full Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility
title_fullStr Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility
title_full_unstemmed Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility
title_short Papanicolaou-Stained Cytosmear Preparations in the Evaluation of Leucocytospermia: A Tertiary Centre Experience and Assessment of Utility
title_sort papanicolaou stained cytosmear preparations in the evaluation of leucocytospermia a tertiary centre experience and assessment of utility
topic leucocytospermia
infertility
reactive oxygen species
semen
url http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1730846
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